HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ulrich, S. Articles by Ehlers, B. Search for Related Content PubMed PubMed Citation Articles by Ulrich, S. Articles by Ehlers, B. Agricola Articles by Ulrich, S. Articles by Ehlers, B.

Characterization of the DNA polymerase loci of the novel porcine lymphotropic herpesviruses 1 and 2 in domestic and feral pigs

Robert Koch-Institut, Nordufer 20, 13353 Berlin, Germany¹

Author for correspondence: Bernhard Ehlers. Fax +49 30 4547 2598. e-mail ehlersb{at}rki.de

Two novel porcine gammaherpesviruses, porcine lymphotropic herpesviruses 1 and 2 (PLHV-1 and -2), have been detected by amplification of short DNA polymerase (DPOL) sequences from blood and spleen of domestic pigs while searching for unknown herpesviruses in pigs as possible risk factors in xenotransplantation. In the present study, the DPOL genes of the two viruses and the open reading frames (ORFs) that follow in the downstream direction were amplified by PCR-based genome walking from adaptor-ligated restriction fragment libraries of porcine spleen samples. The sequences determined for the two PLHVs exhibited a very low G+C content (37 mol%) and a marked suppression of the CpG dinucleotide frequency. The DPOL proteins encoded were 95% identical and showed a close relationship (60% identity) to the DPOL protein of a ruminant gammaherpesvirus, alcelaphine herpesvirus 1 (AlHV-1). This was confirmed by phylogenetic analyses of the conserved regions of the two PLHV DPOL proteins. The PLHV ORFs downstream of DPOL exhibited 83% identity to each other and >>50% similarity to ORF A5, the position equivalent of AlHV-1. From these data, the PLHVs can be firmly classified to the subfamily Gammaherpesvirinae. To find a natural reservoir for the PLHVs, organs of feral pigs were screened with five different PCR assays, targetting either the DPOL gene or 3'-flanking sequences. In all samples, PLHV sequences were detected that originated predominantly from PLHV-2, suggesting the possibility of virus transfer between feral and domestic pig populations.

This article has been cited by other articles:

				B. Ehlers, J. Kuchler, N. Yasmum, G. Dural, S. Voigt, J. Schmidt-Chanasit, T. Jakel, F.-R. Matuschka, D. Richter, S. Essbauer, et al. Identification of Novel Rodent Herpesviruses, Including the First Gammaherpesvirus of Mus musculus J. Virol., August 1, 2007; 81(15): 8091 - 8100. [Abstract] [Full Text] [PDF]

				B. Ehlers, G. Dural, M. Marschall, V. Schregel, M. Goltz, and J. Hentschke Endotheliotropic elephant herpesvirus, the first betaherpesvirus with a thymidine kinase gene. J. Gen. Virol., October 1, 2006; 87(Pt 10): 2781 - 2789. [Abstract] [Full Text] [PDF]

				B. Ehlers and S. Lowden Novel herpesviruses of Suidae: indicators for a second genogroup of artiodactyl gammaherpesviruses J. Gen. Virol., April 1, 2004; 85(4): 857 - 862. [Abstract] [Full Text] [PDF]

				S. Albini, W. Zimmermann, F. Neff, B. Ehlers, H. Hani, H. Li, D. Hussy, M. Engels, and M. Ackermann Identification and Quantification of Ovine Gammaherpesvirus 2 DNA in Fresh and Stored Tissues of Pigs with Symptoms of Porcine Malignant Catarrhal Fever J. Clin. Microbiol., February 1, 2003; 41(2): 900 - 904. [Abstract] [Full Text] [PDF]

				B. Ehlers, S. Burkhardt, M. Goltz, V. Bergmann, A. Ochs, H. Weiler, and J. Hentschke Genetic and ultrastructural characterization of a European isolate of the fatal endotheliotropic elephant herpesvirus J. Gen. Virol., March 1, 2001; 82(3): 475 - 482. [Abstract] [Full Text]

				C. A. Huang, Y. Fuchimoto, Z. L. Gleit, T. Ericsson, A. Griesemer, R. Scheier-Dolberg, E. Melendy, H. Kitamura, J. A. Fishman, J. A. Ferry, et al. Posttransplantation lymphoproliferative disease in miniature swine after allogeneic hematopoietic cell transplantation: similarity to human PTLD and association with a porcine gammaherpesvirus Blood, March 1, 2001; 97(5): 1467 - 1473. [Abstract] [Full Text] [PDF]

				W. Zimmermann, H. Broll, B. Ehlers, H.-J. Buhk, A. Rosenthal, and M. Goltz Genome Sequence of Bovine Herpesvirus 4, a Bovine Rhadinovirus, and Identification of an Origin of DNA Replication J. Virol., February 1, 2001; 75(3): 1186 - 1194. [Abstract] [Full Text]

				S. Bösch, C. Arnauld, and A. Jestin Study of Full-Length Porcine Endogenous Retrovirus Genomes with Envelope Gene Polymorphism in a Specific-Pathogen-Free Large White Swine Herd J. Virol., September 15, 2000; 74(18): 8575 - 8581. [Abstract] [Full Text]