J Gen Virol Faster Access
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bello, L. J.
Right arrow Articles by Barklie Clements, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bello, L. J.
Right arrow Articles by Barklie Clements, J.
Agricola
Right arrow Articles by Bello, L. J.
Right arrow Articles by Barklie Clements, J.
Journal of General Virology (1999), 80, 3207-3215.
© 1999 Society for General Microbiology

Animal: DNA Viruses

The human herpesvirus-8 ORF 57 gene and its properties

Leonard J. Bello1,2, Andrew J. Davison1, Mark A. Glenn3, Adrian Whitehouse4, Nikki Rethmeier1, Thomas F. Schulz3 and J. Barklie Clements1

Institute of Virology, University of Glasgow, Church Street, Glasgow G11 5JR, UK1
Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA2
Department of Medical Microbiology, University of Liverpool, UK3
Molecular Medicine Unit, St James University Hospital, Leeds, UK4

Author for correspondence: J. Barklie Clements. Fax +44 141 337 2236. e-mail b.clements{at}vir.gla.ac.uk

Human herpesvirus-8 (HHV-8) is a {gamma}2 lymphotropic herpesvirus associated with Kaposi’s sarcoma, a major neoplasm of AIDS patients, and with other AIDS-related neoplasms. The HHV-8 ORF 57 gene is conserved throughout the herpesvirus family and has a herpes simplex virus type 1 homologue, IE63 (also termed ICP27), which is an essential regulatory protein and acts at both transcriptional and post-transcriptional levels. We show that, contrary to the published HHV-8 sequence, which predicts a protein of 275 amino acids, the ORF 57 gene is spliced, contains a single intron and encodes a protein of 455 amino acids. For several gammaherpesviruses examined, the upstream coding exon is 16–17 amino acids in length and is rich in methionine residues. When ORF 57 was fused to the gene for enhanced green fluorescent protein (EGFP), the fusion protein exhibited a punctate nuclear distribution that co-localized with the cellular splicing factor SC-35. Unlike the IE63–EGFP fusion protein, ORF 57–EGFP did not shuttle from the nucleus to the cytoplasm in the presence of actinomycin D. However, ORF 57–EGFP was capable of shuttling from a transfected monkey nucleus to a recipient mouse nucleus in an interspecies heterokaryon assay. These data indicate that HHV-8 ORF 57 and IE63 possess certain common properties.




This article has been cited by other articles:


Home page
 J. Virol.Home page
G. Sander, A. Konrad, M. Thurau, E. Wies, R. Leubert, E. Kremmer, H. Dinkel, T. Schulz, F. Neipel, and M. Sturzl
Intracellular Localization Map of Human Herpesvirus 8 Proteins
J. Virol., February 15, 2008; 82(4): 1908 - 1922.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
D. Palmeri, S. Spadavecchia, K. D. Carroll, and D. M. Lukac
Promoter- and Cell-Specific Transcriptional Transactivation by the Kaposi's Sarcoma-Associated Herpesvirus ORF57/Mta Protein
J. Virol., December 15, 2007; 81(24): 13299 - 13314.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M. Nekorchuk, Z. Han, T.-T. Hsieh, and S. Swaminathan
Kaposi's Sarcoma-Associated Herpesvirus ORF57 Protein Enhances mRNA Accumulation Independently of Effects on Nuclear RNA Export
J. Virol., September 15, 2007; 81(18): 9990 - 9998.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
Z. Han, E. Marendy, Y.-D. Wang, J. Yuan, J. T. Sample, and S. Swaminathan
Multiple Roles of Epstein-Barr Virus SM Protein in Lytic Replication
J. Virol., April 15, 2007; 81(8): 4058 - 4069.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
Z. Han and S. Swaminathan
Kaposi's Sarcoma-Associated Herpesvirus Lytic Gene ORF57 Is Essential for Infectious Virion Production.
J. Virol., June 1, 2006; 80(11): 5251 - 5260.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J. A. Schwartz, E. E. Brittle, A. E. Reynolds, L. W. Enquist, and S. J. Silverstein
UL54-Null Pseudorabies Virus Is Attenuated in Mice but Productively Infects Cells in Culture
J. Virol., January 15, 2006; 80(2): 769 - 784.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J. Wang, J. Zhang, L. Zhang, W. Harrington Jr., J. T. West, and C. Wood
Modulation of Human Herpesvirus 8/Kaposi's Sarcoma-Associated Herpesvirus Replication and Transcription Activator Transactivation by Interferon Regulatory Factor 7
J. Virol., February 15, 2005; 79(4): 2420 - 2431.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
P. Malik and J. B. Clements
Protein kinase CK2 phosphorylation regulates the interaction of Kaposi's sarcoma-associated herpesvirus regulatory protein ORF57 with its multifunctional partner hnRNP K
Nucleic Acids Res., October 14, 2004; 32(18): 5553 - 5569.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
P. Malik, D. J. Blackbourn, M. F. Cheng, G. S. Hayward, and J. B. Clements
Functional co-operation between the Kaposi's sarcoma-associated herpesvirus ORF57 and ORF50 regulatory proteins
J. Gen. Virol., August 1, 2004; 85(8): 2155 - 2166.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
P. Malik, D. J. Blackbourn, and J. B. Clements
The Evolutionarily Conserved Kaposi's Sarcoma-associated Herpesvirus ORF57 Protein Interacts with REF Protein and Acts as an RNA Export Factor
J. Biol. Chem., July 30, 2004; 279(31): 33001 - 33011.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
V. Ruvolo, L. Sun, K. Howard, S. Sung, H.-J. Delecluse, W. Hammerschmidt, and S. Swaminathan
Functional Analysis of Epstein-Barr Virus SM Protein: Identification of Amino Acids Essential for Structure, Transactivation, Splicing Inhibition, and Virion Production
J. Virol., January 1, 2004; 78(1): 340 - 352.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
C. Cunningham, S. Barnard, D. J. Blackbourn, and A. J. Davison
Transcription mapping of human herpesvirus 8 genes encoding viral interferon regulatory factors
J. Gen. Virol., June 1, 2003; 84(6): 1471 - 1483.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
E. Caselli, M. Galvan, F. Santoni, A. Rotola, A. Caruso, E. Cassai, and D. D. Luca
Human herpesvirus-8 (Kaposi's sarcoma-associated virus) ORF50 increases in vitro cell susceptibility to human immunodeficiency virus type 1 infection
J. Gen. Virol., May 1, 2003; 84(5): 1123 - 1131.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
V. Ruvolo, L. Navarro, C. E. Sample, M. David, S. Sung, and S. Swaminathan
The Epstein-Barr Virus SM Protein Induces STAT1 and Interferon-Stimulated Gene Expression
J. Virol., March 15, 2003; 77(6): 3690 - 3701.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J. Poppers, M. Mulvey, C. Perez, D. Khoo, and I. Mohr
Identification of a Lytic-Cycle Epstein-Barr Virus Gene Product That Can Regulate PKR Activation
J. Virol., December 6, 2002; 77(1): 228 - 236.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J. L. Boyer, S. Swaminathan, and S. J. Silverstein
The Epstein-Barr Virus SM Protein Is Functionally Similar to ICP27 from Herpes Simplex Virus in Viral Infections
J. Virol., August 12, 2002; 76(18): 9420 - 9433.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
E. Caselli, P. Menegazzi, A. Bracci, M. Galvan, E. Cassai, and D. Di Luca
Human herpesvirus-8 (Kaposi's sarcoma-associated herpesvirus) ORF50 interacts synergistically with the tat gene product in transactivating the human immunodeficiency virus type 1 LTR
J. Gen. Virol., August 1, 2001; 82(8): 1965 - 1970.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
V. Ruvolo, A. K. Gupta, and S. Swaminathan
Epstein-Barr Virus SM Protein Interacts with mRNA In Vivo and Mediates a Gene-Specific Increase in Cytoplasmic mRNA
J. Virol., July 1, 2001; 75(13): 6033 - 6041.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M Neumann, E Afonina, F Ceccherini-Silberstein, S Schlicht, V Erfle, G. Pavlakis, and R Brack-Werner
Nucleocytoplasmic transport in human astrocytes: decreased nuclear uptake of the HIV Rev shuttle protein
J. Cell Sci., January 5, 2001; 114(9): 1717 - 1729.
[Abstract] [PDF]

Home page
 J. Virol.Home page
R. Peng, J. Tan, and P. D. Ling
Conserved Regions in the Epstein-Barr Virus Leader Protein Define Distinct Domains Required for Nuclear Localization and Transcriptional Cooperation with EBNA2
J. Virol., November 1, 2000; 74(21): 9953 - 9963.
[Abstract] [Full Text]

Home page
 J. Gen. Virol.Home page
D. J. Goodwin, K. T. Hall, M. S. Giles, M. A. Calderwood, A. F. Markham, and A. Whitehouse
The carboxy terminus of the herpesvirus saimiri ORF 57 gene contains domains that are required for transactivation and transrepression
J. Gen. Virol., September 1, 2000; 81(9): 2253 - 2265.
[Abstract] [Full Text]

Home page
 J. Virol.Home page
S. M. Bunnell and S. A. Rice
The Conserved Carboxyl-Terminal Half of Herpes Simplex Virus Type 1 Regulatory Protein ICP27 Is Dispensable for Viral Growth in the Presence of Compensatory Mutations
J. Virol., August 15, 2000; 74(16): 7362 - 7374.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1999 by the Society for General Microbiology.