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Identification of a new promoter in the early region of the human papillomavirus type 16 genome

Institute of Molecular Pathology, The Protein Laboratory, University of Copenhagen, Panum Institute, Bldg 6.2, Blegdamsvej 3C, DK-2200 Copenhagen N, Denmark¹

Author for correspondence: Bodil Norrild. Fax +45 3536 0116. e-mail bono{at}biobase.dk

Transcription of the human papillomavirus type 16 (HPV-16) genome is controlled by several promoters; the P₉₇ promoter is considered to be the main one. An additional promoter has been identified within the E7 ORF as well as an antisense promoter just upstream of the L2 ORF. The significance of these promoters for early and late gene expression and their activity related to cell differentiation is not known in detail. Identification of two new, previously undescribed transcription start sites at nt 542 just upstream of the E7 ORF and at nt 611 within the E7 ORF is reported. The promoter responsible for the start site at nt 542 (P₅₄₂) was active in SiHa, HeLa and C33A cells. Very low promoter activity was found upstream of the nt 611 start site. The E7 protein has previously been shown to be synthesized from a polycistronic mRNA encoding both the E6 and E7 proteins under the control of the P₉₇ promoter. The data reported in the present paper suggest that promoter P₅₄₂ may control synthesis of the E7 oncoprotein from a monocistronic mRNA.

This article has been cited by other articles:

				J. A. Glahder, C. N. Hansen, J. Vinther, B. S. Madsen, and B. Norrild A promoter within the E6 ORF of human papillomavirus type 16 contributes to the expression of the E7 oncoprotein from a monocistronic mRNA J. Gen. Virol., December 1, 2003; 84(12): 3429 - 3441. [Abstract] [Full Text] [PDF]

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