Journal of General Virology, Vol 80, 277-282, Copyright © 1999 by Society for General Microbiology

ARTICLES

Identification of neutralizing epitopes on a European strain of swine vesicular disease virus

SK Nijhar, DK Mackay, E Brocchi, NP Ferris, RP Kitching and NJ Knowles
Institute for Animal Health, Pirbright Laboratory, Surrey, UK.

Six neutralizing monoclonal antibodies (MAbs) were used to isolate MAb neutralization-resistant (MAR) mutants from a recent European strain of swine vesicular disease virus (SVDV), ITL/9/93. Sequencing of MAR mutants identified two epitopes located at positions analogous to sites 2A (VP2) and 3B (VP3) on poliovirus (PV) which have been previously identified on a Japanese strain of SVDV. A third epitope near to the C terminus of VP1, not previously recognized on SVDV, was tentatively identified in a region analogous to site 1 of PV. A fourth epitope, located in the C-terminal region of VP3, has never before been recognized as a site of neutralization on picornaviruses. All four epitopes were predicted to be surface-exposed.

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