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Journal of General Virology, Vol 80, 437-440, Copyright © 1999 by Society for General Microbiology

ARTICLES

The R27080 glycoprotein is abundantly secreted from human cytomegalovirus-infected fibroblasts

J Mullberg, ML Hsu, CT Rauch, MJ Gerhart, A Kaykas and D Cosman
Immunex Corporation, Seattle, WA 98101, USA. mullberg@mail.uni-mainz.de

A 45 kDa glycoprotein was purified from the culture media of human cytomegalovirus (HCMV)-infected fibroblasts. N-terminal sequencing revealed that the protein, R27080, is the translation product of the R27080 open reading frame of HCMV. R27080 is highly glycosylated and contains no cysteine or methionine residues. Proteolytic cleavage of R27080 by a furin-like enzyme was analysed in transfected COS-7 cells. R27080 is the first identified viral protein secreted from HCMV- infected cells.


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