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Journal of General Virology, Vol 80, 543-547, Copyright © 1999 by Society for General Microbiology
ARTICLES |
D Zipeto, B Bodaghi, L Laurent, JL Virelizier and S Michelson
Unite d'Immunologie Virale, Institut Pasteur, Paris, France. zipeto@pasteur.fr
In permissive cells, human cytomegalovirus encodes the protein US28, a functional CC chemokine receptor. US28 polyadenylated mRNA could be detected by RT-PCR as early as 2 h post-infection. US28 mRNA appeared after major IE1 transcripts (UL123), but before transcripts of the early genes pp65 (UL83) and gB (UL55), and the late gene pp150 (UL32). This temporal appearance indicates that US28 is transcribed earlier than previously reported. Furthermore, US28 mRNA could be detected in semi- and non-permissive cells.
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