J Gen Virol Faster Access
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ayllon, M. A.
Right arrow Articles by Moreno, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ayllon, M. A.
Right arrow Articles by Moreno, P.
Agricola
Right arrow Articles by Ayllon, M. A.
Right arrow Articles by Moreno, P.

Journal of General Virology, Vol 80, 817-821, Copyright © 1999 by Society for General Microbiology

ARTICLES

New defective RNAs from citrus tristeza virus: evidence for a replicase- driven template switching mechanism in their generation

MA Ayllon, C Lopez, J Navas-Castillo, M Mawassi, WO Dawson, J Guerri, R Flores and P Moreno
Instituto Valenciano de Investigaciones Agrarias, Moncada, Spain.

Defective RNAs (D-RNAs) ranging in size from 1968 to 2759 nt were detected in four citrus tristeza virus (CTV) isolates by hybridization of electroblotted dsRNAs with two probes specific for the 5'- and 3'- terminal genomic regions. The RNAs that hybridized with both probes were eluted, cloned and sequenced. Comparison with the sequences of the corresponding genomic regions of the helper virus showed, in all cases, over 99% nucleotide identity and direct repeats of 4-5 nt flanking or in the vicinity of the junction sites. The presence of the repeats from two separate genome locations suggests a replicase-driven template switching mechanism for the generation of these CTV D-RNAs. Two of the CTV isolates that differed greatly in their pathogenicity contained an identical D-RNA, suggesting that it is unlikely that this D-RNA is involved in symptom modulation, which may be caused by another factor.


This article has been cited by other articles:


Home page
 J. Gen. Virol.Home page
S. Martin, A. Sambade, L. Rubio, M. C. Vives, P. Moya, J. Guerri, S. F. Elena, and P. Moreno
Contribution of recombination and selection to molecular evolution of Citrus tristeza virus
J. Gen. Virol., June 1, 2009; 90(6): 1527 - 1538.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
J. C. K. Ng, T. Tian, and B. W. Falk
Quantitative parameters determining whitefly (Bemisia tabaci) transmission of Lettuce infectious yellows virus and an engineered defective RNA
J. Gen. Virol., September 1, 2004; 85(9): 2697 - 2707.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
C.-P. Cheng and P. D. Nagy
Mechanism of RNA Recombination in Carmo- and Tombusviruses: Evidence for Template Switching by the RNA-Dependent RNA Polymerase In Vitro
J. Virol., November 15, 2003; 77(22): 12033 - 12047.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
L. Rubio, M. A. Ayllon, P. Kong, A. Fernandez, M. Polek, J. Guerri, P. Moreno, and B. W. Falk
Genetic Variation of Citrus Tristeza Virus Isolates from California and Spain: Evidence for Mixed Infections and Recombination
J. Virol., September 1, 2001; 75(17): 8054 - 8062.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1999 by the Society for General Microbiology.