Journal of General Virology, Vol 80, 1133-1139, Copyright © 1999 by Society for General Microbiology

ARTICLES

Potyvirus helper component-proteinase and coat protein (CP) have coordinated functions in virus--host interactions and the same CP motif affects virus transmission and accumulation

J Andrejeva, U Puur, A Merits, F Rabenstein, L Jarvekulg and JPT Valkonen
Institute of Biotechnology, PO Box 56, Viikki Biocenter, FIN-00014 University of Helsinki, Finland

Amino acid differences between helper component-proteinase (HC-Pro) and coat protein (CP) that are putatively associated with biological differences between isolates PVA-B11 and PVA-U of potato A potyvirus (PVA) were studied using an infectious cDNA clone of PVA-B11. Replacement of the entire CP gene of PVA-B11 with the CP gene of PVA-U reduced virus accumulation in tobacco 5-fold, to the level of PVA-U. In contrast, four simultaneous amino acid substitutions made in PVA-B11 HC-Pro (according to PVA-U HC-Pro) increased virus accumulation 2- to 4-fold. A single substitution (S7G) at the CP N terminus reduced virus accumulation 10-fold, but restored aphid-transmissibility of PVA-B11. Simultaneous mutation of HC-Pro and replacement of CP in B11 delayed systemic movementin tobacco and limited cell-to-cell movement in potato. These results imply coordinated functions of HC-Pro and CP in accumulation and movement of PVA, because the phenotypic effects caused by simultaneous mutation of the two genes were different from the expected 'sum' of phenotypic changes observed following mutation of only one gene at a time.

This article has been cited by other articles:

				C. M. Wallis, A. L. Stone, D. J. Sherman, V. D. Damsteegt, F. E. Gildow, and W. L. Schneider Adaptation of plum pox virus to a herbaceous host (Pisum sativum) following serial passages J. Gen. Virol., October 1, 2007; 88(10): 2839 - 2845. [Abstract] [Full Text] [PDF]

				V. Paalme, E. Gammelgard, L. Jarvekulg, and J. P. T. Valkonen In vitro recombinants of two nearly identical potyviral isolates express novel virulence and symptom phenotypes in plants J. Gen. Virol., March 1, 2004; 85(3): 739 - 747. [Abstract] [Full Text] [PDF]

				P. Puustinen, M.-L. Rajamaki, K. I. Ivanov, J. P. T. Valkonen, and K. Makinen Detection of the Potyviral Genome-Linked Protein VPg in Virions and Its Phosphorylation by Host Kinases J. Virol., November 13, 2002; 76(24): 12703 - 12711. [Abstract] [Full Text] [PDF]

				E. I. Savenkov and J. P. T. Valkonen Silencing of a viral RNA silencing suppressor in transgenic plants J. Gen. Virol., September 1, 2002; 83(9): 2325 - 2335. [Abstract] [Full Text] [PDF]

				G. Roudet-Tavert, S. German-Retana, T. Delaunay, B. Delecolle, T. Candresse, and O. Le Gall Interaction between potyvirus helper component-proteinase and capsid protein in infected plants J. Gen. Virol., June 1, 2002; 83(7): 1765 - 1770. [Abstract] [Full Text] [PDF]

				A. Merits, M.-L. Rajamaki, P. Lindholm, P. Runeberg-Roos, T. Kekarainen, P. Puustinen, K. Makelainen, J. P. T. Valkonen, and M. Saarma Proteolytic processing of potyviral proteins and polyprotein processing intermediates in insect and plant cells J. Gen. Virol., May 1, 2002; 83(5): 1211 - 1221. [Abstract] [Full Text] [PDF]

				L. A. Baratova, A. V. Efimov, E. N. Dobrov, N. V. Fedorova, R. Hunt, G. A. Badun, A. L. Ksenofontov, L. Torrance, and L. Jarvekulg In Situ Spatial Organization of Potato Virus A Coat Protein Subunits as Assessed by Tritium Bombardment J. Virol., October 15, 2001; 75(20): 9696 - 9702. [Abstract] [Full Text] [PDF]

				T. Arazi, Y. M. Shiboleth, and A. Gal-On A Nonviral Peptide Can Replace the Entire N Terminus of Zucchini Yellow Mosaic Potyvirus Coat Protein and Permits Viral Systemic Infection J. Virol., July 15, 2001; 75(14): 6329 - 6336. [Abstract] [Full Text]