Journal of General Virology, Vol 80, 1157-1165, Copyright © 1999 by Society for General Microbiology

ARTICLES

Localization of pestiviral envelope proteins Erns and E2 at the cell surface and on isolated particles

F Weil, E Weil, G Unger, A Saalmuller and HG Thiel
Federal Research Centre for Virus Diseases of Animals, Paul-Ehrlich-Strasse 28, D-72076 Tubingen, Germany

The glycoproteins Erns of classical swine fever virus (CSFV) and Erns and E2 of bovine viral diarrhoea virus (BVDV) are shown to be located at the surface of infected cells bythe use of indirect immunofluorescence and by cytofluorometric analysis. The positive immunostaining of the cell surface was further analysed by immunogold electron microscopy and it could be shown that only extracellular virions were labelled. Gold granules were not seen at the cellular plasma membrane. In contrast to BVDV E2, the CSFV E2 of virions sticking to the plasma membrane was not accessible to the respective monoclonal antibodies. However, CSFV particles isolated from culture supernatant were able to bind both monoclonal anti-Erns and anti-E2 antibodies. For CSFV and BVDV, binding of anti-Erns antibodies to the virions was more pronounced than that of anti-E2. This finding was unexpected since E2 is considered to be the immunodominant glycoprotein.

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