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Journal of General Virology, Vol 80, 1725-1733, Copyright © 1999 by Society for General Microbiology


ARTICLES

Nucleotide sequences and further characterization of human papillomavirus DNA present in the CaSki, SiHa and HeLa cervical carcinoma cell lines

JD Meissner
Human Papillomavirus Section, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, US Department of Health and Human Services, 1600 Clifton Road NE, Atlanta, Georgia 30333, USA

The complete nucleotide sequences of the human papillomavirus type 16 (HPV-16) variants present in the CaSki and SiHa cervical carcinoma cell lines and the primary subgenomic HPV-18 variant present in the HeLa cervical carcinoma cell line were determined using overlapping bulk PCR products as templates. PCR-based methods were also used to characterize five previously unreported CaSki HPV-16 genomic disruptions and the 5' cellular--viral junction common to all HeLa HPV-18 subgenomic structures.


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