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Mapping of domains on the human parainfluenza virus type 2 nucleocapsid protein (NP) required for NP–phosphoprotein or NP–NP interaction

Department of Microbiology, Mie University School of Medicine, 2-174, Edobashi, Tsu-Shi, Mie-Ken, 514-8507, Japan¹

Author for correspondence: Machiko Nishio.Fax +81 59 231 5008. e-mail nishio{at}doc.medic.mie-u.ac.jp

The epitopes recognized by 41 monoclonal antibodies directed against the nucleocapsid protein (NP) of human parainfluenza virus type 2 (hPIV-2) were mapped on the primary structure of the hPIV-2 NP protein by testing their reactivities with deletion mutants. By Western immunoblotting using these monoclonal antibodies, the analysis of deletion mutants of the hPIV-2 NP protein was performed to identify the region essential for NP–NP interaction and phosphoprotein (P)-binding sites on the NP protein. The results indicate that the N-terminal 294 aa of the NP protein are all required for NP–NP self-assembly, and that two C-terminal parts of the NP protein are essential for NP–P binding: one region, aa 295–402, is required for binding to the C-terminal part of the P protein and another region, aa 403–494, to the N-terminal part of the P protein.

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