| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Animal: DNA Viruses |
Institute of Virology, Universität zu Köln, Fürst-Pückler Str. 56, 50935 Köln, Germany1
Author for correspondence: Herbert Pfister.Fax +49 221 4783902. e-mail Herbert.Pfister{at}medizin.uni-koeln.de
Transcription of oncogenes E6 and E7 of human papillomavirus type 16 (HPV-16) from the P97 promoter is regulated by viral and cellular proteins. The transcription factor YY1 represses transcription through binding to cognate sequences in the long control region (LCR). In HPV-16 DNA from cervical carcinomas, mutations of YY1-binding sites have been identified that increase P97 activity 3–6-fold. A second, SP1-binding site has now been identified in the HPV-16 LCR (nt 7842–7847), which overlaps the YY1-binding site at positions 7840–7848. A point mutation within this YY1 site in viral DNA from a cervical cancer, previously shown to prevent YY1 binding, was shown to increase SP1 binding and P97 activity 4·7-fold. An engineered mutant eliminating SP1 binding showed only 1- to 1·6-fold increased P97 activity. It is concluded that competition between SP1 and YY1 for DNA binding plays a major role in YY1 repression mediated by the binding site at positions 7840–7848.
This article has been cited by other articles:
|
|
 |
|
  R. Jia, X. Liu, M. Tao, M. Kruhlak, M. Guo, C. Meyers, C. C. Baker, and Z.-M. Zheng Control of the Papillomavirus Early-to-Late Switch by Differentially Expressed SRp20 J. Virol., January 1, 2009; 83(1): 167 - 180. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Pande, N. Jain, B. K. Prusty, S. Bhambhani, S. Gupta, R. Sharma, S. Batra, and B. C. Das Human Papillomavirus Type 16 Variant Analysis of E6, E7, and L1 Genes and Long Control Region in Biopsy Samples from Cervical Cancer Patients in North India J. Clin. Microbiol., March 1, 2008; 46(3): 1060 - 1066. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Dai, Z. Zhang, J. Bick, and R. N. Beachy Essential role of the Box II cis element and cognate host factors in regulating the promoter of Rice tungro bacilliform virus. J. Gen. Virol., March 1, 2006; 87(Pt 3): 715 - 722. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. G. Hubert Variant Upstream Regulatory Region Sequences Differentially Regulate Human Papillomavirus Type 16 DNA Replication throughout the Viral Life Cycle J. Virol., May 15, 2005; 79(10): 5914 - 5922. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Her, R. Claycomb, T. C. Tai, and D. L. Wong Regulation of the Rat Phenylethanolamine N-Methyltransferase Gene by Transcription Factors Sp1 and MAZ Mol. Pharmacol., November 1, 2003; 64(5): 1180 - 1188. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Ai, J. Narahari, and A. Roman Yin Yang 1 Negatively Regulates the Differentiation-Specific E1 Promoter of Human Papillomavirus Type 6 J. Virol., June 1, 2000; 74(11): 5198 - 5205. [Abstract] [Full Text]
|
|
|
|
 |
|
 P. T. La Celle and R. R. Polakowska Human Homeobox HOXA7 Regulates Keratinocyte Transglutaminase Type 1 and Inhibits Differentiation J. Biol. Chem., August 24, 2001; 276(35): 32844 - 32853. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
