HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Wilcock, D. Articles by Smith, G. L. Search for Related Content PubMed PubMed Citation Articles by Wilcock, D. Articles by Smith, G. L. Agricola Articles by Wilcock, D. Articles by Smith, G. L.

The vaccinia virus A40R gene product is a nonstructural, type II membrane glycoprotein that is expressed at the cell surface

Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford OX1 3RE, UK¹
Department of Microbiology and Molecular Genetics, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA²

Author for correspondence: Geoffrey L. Smith.Fax +44 1865 275501. e-mail glsmith{at}molbiol.ox.ac.uk

Gene A40R from vaccinia virus (VV) strain Western Reserve has been characterized. The open reading frame (ORF) was predicted to encode a 159 amino acid, 18152 Da protein with amino acid similarity to C-type animal lectins and to the VV A34R protein, a component of extracellular enveloped virus (EEV). Northern blotting and S1 nuclease mapping showed that gene A40R is transcribed early during infection from a position 12 nucleotides upstream of the ORF, producing a transcript of approximately 600 nucleotides. Rabbit anti-sera were raised against bacterial fusion proteins containing parts of the A40R protein. These were used to identify an 18 kDa primary translation product and N- and O-glycosylated forms of 28, 35 and 38 kDa. The A40R proteins were detected early during infection, formed higher molecular mass complexes under non-reducing conditions and were present on the cell surface but absent from virions. The proteins partitioned with integral membrane proteins in Triton X-114. Canine pancreatic microsomal membranes protected in vitro-translated A40R from proteinase K digestion, suggesting the A40R protein has type II membrane topology. A mutant virus with the A40R gene disrupted after amino acid 50, so as to remove the entire lectin-like domain, and a revertant virus were constructed. Disruption of the A40R gene did not affect virus plaque size, in vitro growth rate and titre, EEV formation, or virus virulence in a murine intranasal model.

This article has been cited by other articles:

				N. Jacobs, R. A.-J. Chen, C. Gubser, P. Najarro, and G. L. Smith Intradermal immune response after infection with Vaccinia virus. J. Gen. Virol., May 1, 2006; 87(Pt 5): 1157 - 1161. [Abstract] [Full Text] [PDF]

				S. Palacios, L. H. Perez, S. Welsch, S. Schleich, K. Chmielarska, F. Melchior, and J. K. Locker Quantitative SUMO-1 Modification of a Vaccinia Virus Protein Is Required for Its Specific Localization and Prevents Its Self-Association Mol. Biol. Cell, June 1, 2005; 16(6): 2822 - 2835. [Abstract] [Full Text] [PDF]

				C. L. Afonso, G. Delhon, E. R. Tulman, Z. Lu, A. Zsak, V. M. Becerra, L. Zsak, G. F. Kutish, and D. L. Rock Genome of Deerpox Virus J. Virol., January 15, 2005; 79(2): 966 - 977. [Abstract] [Full Text] [PDF]

				D. C. Tscharke, P. C. Reading, and G. L. Smith Dermal infection with vaccinia virus reveals roles for virus proteins not seen using other inoculation routes J. Gen. Virol., August 1, 2002; 83(8): 1977 - 1986. [Abstract] [Full Text] [PDF]

				S. Voigt, G. R. Sandford, L. Ding, and W. H. Burns Identification and Characterization of a Spliced C-Type Lectin-Like Gene Encoded by Rat Cytomegalovirus J. Virol., January 15, 2001; 75(2): 603 - 611. [Abstract] [Full Text]

				C. L. Afonso, E. R. Tulman, Z. Lu, L. Zsak, G. F. Kutish, and D. L. Rock The Genome of Fowlpox Virus J. Virol., April 15, 2000; 74(8): 3815 - 3831. [Abstract] [Full Text]