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Journal of General Virology (1999), 80, 2353-2359.
© 1999 Society for General Microbiology

Animal: RNA Viruses

Isolation and characterization of a transfectant influenza B virus altered in RNA segment 6

Kate V. Rowley1, Ruth Harvey1 and Wendy S. Barclay1

School of Animal and Microbial Sciences, University of Reading, Whiteknights, PO Box 228, Reading RG6 6AJ, UK1

Author for correspondence: Wendy Barclay.Fax +44 1189 316671. e-mail w.s.barclay{at}reading.ac.uk

This report describes the successful generation of an influenza B transfectant virus altered in RNA segment 6, which encodes the neuraminidase (NA) protein. The procedure for selection of the transfectant virus relies on the use of strain-specific anti-NA monoclonal antibodies to inhibit growth of the helper virus within the system. A transfectant virus has been engineered which has a coding change in the NA protein. This change resulted in attenuated growth in vitro that could be rescued by addition of exogenous bacterial NA. The mutant virus-associated NA activity was unstable as a result of the engineered changes. The ability to genetically manipulate influenza B virus segment 6 will allow us to assess the function of both NA and the small protein NB, also coded from this RNA, within the context of the virus infectious cycle.




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F. T. Vreede, H. Gifford, and G. G. Brownlee
Role of Initiating Nucleoside Triphosphate Concentrations in the Regulation of Influenza Virus Replication and Transcription
J. Virol., July 15, 2008; 82(14): 6902 - 6910.
[Abstract] [Full Text] [PDF]


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