| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Animal: RNA Viruses |
Department of Pathology and Microbiology,1 AVC Inc.2 and Department of Health Management3, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PEI, Canada
Author for correspondence: Frederick Kibenge. Fax +1 902 566 0851. e-mail kibenge{at}upei.ca
Infectious salmon anaemia virus (ISAV) is a new orthomyxovirus-like virus. Thirteen isolates of ISAV (11 from Canada, one from Norway and one from Scotland) were studied for their replication in the CHSE-214 cell line compared with that in the SHK-1 cell line. All isolates replicated in SHK-1 cells, producing CPE between 3 and 12 days post-inoculation (p.i.). Six Canadian isolates also replicated in CHSE-214 cells, with production of CPE between 4 and 17 days p.i. Analysis of a one-step growth curve of ISAV in CHSE-214 cells showed that progeny virions remained predominantly cell-associated, accounting for the focalized nature of the CPE in the cell monolayer. One isolate (HKS 36) replicated in CHSE-214 cells, as shown by positive RT–PCR results of blind passages, but was non-cytopathic. All of the isolates were analysed for genetic heterogeneity by RT–PCR and RFLP with EcoRI and XhoI in a fraction of genome segment 2. The Canadian isolates showed a different RFLP profile to those of isolates Glesvaer/2/90 from Norway and 390/98 from Scotland. Structural proteins of four isolates, ‘Back Bay 98’, RPC/NB-877, RPC/NB-049 and Glesvaer/2/90, were examined further by SDS–PAGE. All viruses showed four major polypeptides, designated here as VP1–VP4, in Coomassie blue-stained gels. In isolates Glesvaer/2/90 and RPC/NB-877, these viral proteins had estimated molecular masses of 74, 53, 46 and 26·5 kDa, respectively. Viral proteins in isolates ‘Back Bay 98’ and RPC/NB-049 were of similar sizes, except that VP3 was 43 kDa. Taken together, these results show that there are phenotypic differences among strains of ISAV.
This article has been cited by other articles:
|
|
 |
|
  R. C. Cipriano Antibody against infectious salmon anaemia virus among feral Atlantic salmon (Salmo salar) ICES J. Mar. Sci., June 1, 2009; 66(5): 865 - 870. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. G. Olveira, F. Soares, S. Engrola, C. P. Dopazo, and I. Bandin Antemortem versus postmortem methods for detection of betanodavirus in Senegalese sole (Solea senegalensis) J Vet Diagn Invest, March 1, 2008; 20(2): 215 - 219. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. S. B. Kibenge, M. J. T. Kibenge, D. Groman, and S. McGeachy In vivo correlates of infectious salmon anemia virus pathogenesis in fish J. Gen. Virol., September 1, 2006; 87(9): 2645 - 2652. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Aspehaug, A. B. Mikalsen, M. Snow, E. Biering, and S. Villoing Characterization of the Infectious Salmon Anemia Virus Fusion Protein J. Virol., October 1, 2005; 79(19): 12544 - 12553. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Moneke, D. B. Groman, G. M. Wright, H. Stryhn, G. R. Johnson, B. O. Ikede, and F. S. B. Kibenge Correlation of Virus Replication in Tissues with Histologic Lesions in Atlantic Salmon Experimentally Infected with Infectious Salmon Anemia Virus Vet. Pathol., May 1, 2005; 42(3): 338 - 349. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Joseph, A. Cepica, L. Brown, B. O. Ikede, and F. S. B. Kibenge Mechanism of cell death during infectious salmon anemia virus infection is cell type-specific J. Gen. Virol., October 1, 2004; 85(10): 3027 - 3036. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Falk, V. Aspehaug, R. Vlasak, and C. Endresen Identification and Characterization of Viral Structural Proteins of Infectious Salmon Anemia Virus J. Virol., March 15, 2004; 78(6): 3063 - 3071. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Joseph, M. T. Kibenge, and F. S. B. Kibenge Antibody-mediated growth of infectious salmon anaemia virus in macrophage-like fish cell lines J. Gen. Virol., July 1, 2003; 84(7): 1701 - 1710. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. C. Clouthier, T. Rector, N. E. C. Brown, and E. D. Anderson Genomic organization of infectious salmon anaemia virus J. Gen. Virol., February 1, 2002; 83(2): 421 - 428. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. S. B. Kibenge, M. J. T. Kibenge, P. K. McKenna, P. Stothard, R. Marshall, R. R. Cusack, and S. McGeachy Antigenic variation among isolates of infectious salmon anaemia virus correlates with genetic variation of the viral haemagglutinin gene J. Gen. Virol., December 1, 2001; 82(12): 2869 - 2879. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Krossoy, M. Devold, L. Sanders, P. M. Knappskog, V. Aspehaug, K. Falk, A. Nylund, S. Koumans, C. Endresen, and E. Biering Cloning and identification of the infectious salmon anaemia virus haemagglutinin J. Gen. Virol., July 1, 2001; 82(7): 1757 - 1765. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
