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Replication of equine herpesvirus type 1 in freshly isolated equine peripheral blood mononuclear cells and changes in susceptibility following mitogen stimulation

Laboratory of Virology, Faculty of Veterinary Medicine, University of Gent, Salisburylaan 133, B-9820 Merelbeke, Belgium¹

Author for correspondence: Hans Nauwynck. Fax +32 9 264 74 95. e-mail hans.nauwynck{at}rug.ac.be

In the present study, the outcome of an inoculation of equine peripheral blood mononuclear cells (PBMC) with equine herpesvirus type 1 (EHV-1) was studied in vitro. Cytoplasmic and plasma membrane expression of viral antigens, intra- and extracellular virus titres, and plaque formation in co-culture were determined. EHV-1 replicated in monocytes, although in a highly restricted way. Viral antigens were found at maximum levels (8·7% of the monocytes) at 12 h post-infection. The infection was productive in 0·16% of the monocytes. The virus yield was 10^0·7 TCID₅₀ per productive cell. In a population of resting lymphocytes, 0·9% of cells were infected and less than 0·05% produced infectious virus. After prestimulation with different mitogens, the number of infected lymphocytes increased four to twelve times. The susceptible lymphocytes were T-lymphocytes. In mitogen-stimulated lymphocytes, clear expression of viral antigens was found on the plasma membrane.

This article has been cited by other articles:

				K. M. van der Meulen, H. J. Nauwynck, and M. B. Pensaert Absence of viral antigens on the surface of equine herpesvirus-1-infected peripheral blood mononuclear cells: a strategy to avoid complement-mediated lysis J. Gen. Virol., January 1, 2003; 84(1): 93 - 97. [Abstract] [Full Text] [PDF]

				K. M. van der Meulen, H. J. Nauwynck, and M. B. Pensaert Mitogen stimulation favours replication of equine herpesvirus-1 in equine blood mononuclear cells by inducing cell proliferation and formation of close intercellular contacts J. Gen. Virol., August 1, 2001; 82(8): 1951 - 1957. [Abstract] [Full Text] [PDF]