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Journal of General Virology (2000), 81, 2485-2490.
© 2000 Society for General Microbiology

Animal: RNA Viruses

Selection and characterization of a BHK-21 cell line resistant to infection by Theiler’s murine encephalomyelitis virus due to a block in virus attachment and entry

Shannon Hertzler1, Mark Trottier2 and Howard L. Lipton2,3

Integrated Graduate Program, Northwestern University Medical School, Chicago, IL, USA1
Department of Neurology, Evanston Hospital, 2650 Ridge Avenue, Evanston, IL 60201, USA2
Departments of Neurology, Microbiology-Immunology, and Biochemistry, Molecular Biology and Cell Biology, Northwestern University, Evanston/Chicago, IL, USA3

Author for correspondence: Howard Lipton (at Evanston Hospital). Fax +1 847 570 1568. e-mail hllipton{at}merle.acns.nwu.edu

A clonal population of BHK-21 cells resistant to infection with the low-neurovirulence BeAn strain of Theiler’s murine encephalomyelitis virus (TMEV) was derived after four cycles of infection and characterized. These cells were resistant to both low- and high-neurovirulence TMEV strains due to a block in virus attachment and entry and not in virus replication, since transfection of these cells with TMEV RNA to bypass the entry step(s) induced virus replication and assembly. The resistance to infection was stable for more than a year, suggesting that it is a heritable property arising from a mutation in the susceptible parent BHK-21 population. This cell line is being used to identify a receptor for TMEV.






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