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Animal: RNA Viruses |
Institute for Animal Health, Compton, Newbury, Berkshire RG20 7NN , UK1
Centro Nacional de Biologia Fundamental, Instituto de Salud Carlos III, Majadahonda, 28220 Madrid, Spain2
NIBSC, Blanche Lane, South Mimms, Potters Bar, Hertfordshire EN6 3QG, UK3
Author for correspondence: Gary Bembridge. Fax +44 1635 577263. e-mail Gary.Bembridge{at}bbsrc.ac.uk
Significant protection against respiratory syncytial virus (RSV) infection was induced in mice vaccinated intramuscularly (i.m.) with DNA encoding the F or G protein of RSV. The amounts of IgG1 of IgG2a antibodies in mice immunized with DNA-G alone were similar. However, the antibody response in mice co-immunized with DNA-G and DNA encoding IL-4 (DNA-IL-4) was strongly biased towards IgG1. In contrast, the antibody response in mice co-immunized with DNA-G and DNA-IL-2, -IL-12 or-IFN-
was biased towards IgG2a. Mice vaccinated with DNA-F either alone or in combination with DNA encoding cytokines developed a predominant RSV-specific IgG2a response, which was most pronounced in mice co-immunized with DNA-F and DNA-IL-12 or -IFN-. Vaccinated mice developed only a slightly enhanced pulmonary inflammatory response following RSV challenge. More significantly, and in contrast to mice scarified with recombinant vaccinia virus expressing the G protein, mice vaccinated i.m. with DNA-G did not develop pulmonary eosinophilia, even when the immune response was biased towards a Th2 response by co-administration of DNA-IL-4.
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