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Animal: DNA Viruses |
Molecular Medicine Unit, University of Leeds, St James’s University Hospital, Leeds LS9 7TF, UK1
Author for correspondence: Adrian Whitehouse. Fax +44 113 2444475. e-mail a.whitehouse{at}leeds.ac.uk
The herpesvirus saimiri (HVS) gene product encoded by ORF73 shares a limited homology with the ORF73 encoded protein of Kaposi’s sarcoma-associated herpesvirus (KSHV). It has recently been shown that the KSHV ORF73 protein is expressed during a latent infection and co-localizes with host cell chromosomes, suggesting that it plays a role in episomal maintenance by tethering viral genomes to host cell chromosomes. At present the role of the HVS ORF73 gene product is unknown. However, the expression of HVS ORF73 in a stably transduced human carcinoma cell line, where the HVS genome persists as a non-integrated circular episome, has recently been shown. In this report, the characterization of the HVS ORF73 protein and the mapping of its functional domains are described. The results suggest that the HVS ORF73 gene encodes a 64 kDa nuclear protein. Moreover, the amino terminus contains two functional nuclear localization signals, whereas the carboxy terminus is required for the distinctive speckled nuclear distribution pattern as observed with both the HVS and KSHV ORF73 proteins.
This article has been cited by other articles:
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  R. Griffiths, S. M. Harrison, S. Macnab, and A. Whitehouse Mapping the minimal regions within the ORF73 protein required for herpesvirus saimiri episomal persistence J. Gen. Virol., November 1, 2008; 89(11): 2843 - 2850. [Abstract] [Full Text] [PDF]
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 R. Griffiths and A. Whitehouse Herpesvirus Saimiri Episomal Persistence Is Maintained via Interaction between Open Reading Frame 73 and the Cellular Chromosome-Associated Protein MeCP2 J. Virol., April 15, 2007; 81(8): 4021 - 4032. [Abstract] [Full Text] [PDF]
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M. Calderwood, R. E. White, R. A. Griffiths, and A. Whitehouse Open reading frame 73 is required for herpesvirus saimiri A11-S4 episomal persistence J. Gen. Virol., October 1, 2005; 86(10): 2703 - 2708. [Abstract] [Full Text] [PDF]
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S. Borah, S. C. Verma, and E. S. Robertson ORF73 of Herpesvirus Saimiri, a Viral Homolog of Kaposi's Sarcoma-Associated Herpesvirus, Modulates the Two Cellular Tumor Suppressor Proteins p53 and pRb J. Virol., October 1, 2004; 78(19): 10336 - 10347. [Abstract] [Full Text] [PDF]
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K. Lan, D. A. Kuppers, S. C. Verma, and E. S. Robertson Kaposi's Sarcoma-Associated Herpesvirus-Encoded Latency-Associated Nuclear Antigen Inhibits Lytic Replication by Targeting Rta: a Potential Mechanism for Virus-Mediated Control of Latency J. Virol., June 15, 2004; 78(12): 6585 - 6594. [Abstract] [Full Text] [PDF]
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M. A. Calderwood, K. T. Hall, D. A. Matthews, and A. Whitehouse The herpesvirus saimiri ORF73 gene product interacts with host-cell mitotic chromosomes and self-associates via its C terminus J. Gen. Virol., January 1, 2004; 85(1): 147 - 153. [Abstract] [Full Text] [PDF]
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R. E. White, M. A. Calderwood, and A. Whitehouse Generation and precise modification of a herpesvirus saimiri bacterial artificial chromosome demonstrates that the terminal repeats are required for both virus production and episomal persistence J. Gen. Virol., December 1, 2003; 84(12): 3393 - 3403. [Abstract] [Full Text] [PDF]
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S. C. Verma and E. S. Robertson ORF73 of Herpesvirus Saimiri Strain C488 Tethers the Viral Genome to Metaphase Chromosomes and Binds to cis-Acting DNA Sequences in the Terminal Repeats J. Virol., December 1, 2003; 77(23): 12494 - 12506. [Abstract] [Full Text] [PDF]
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A. Schafer, D. Lengenfelder, C. Grillhosl, C. Wieser, B. Fleckenstein, and A. Ensser The Latency-Associated Nuclear Antigen Homolog of Herpesvirus Saimiri Inhibits Lytic Virus Replication J. Virol., May 15, 2003; 77(10): 5911 - 5925. [Abstract] [Full Text] [PDF]
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K. T. Hall, M. S. Giles, M. A. Calderwood, D. J. Goodwin, D. A. Matthews, and A. Whitehouse The Herpesvirus Saimiri Open Reading Frame 73 Gene Product Interacts with the Cellular Protein p32 J. Virol., October 11, 2002; 76(22): 11612 - 11622. [Abstract] [Full Text] [PDF]
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C. M. Collins, M. M. Medveczky, T. Lund, and P. G. Medveczky The terminal repeats and latency-associated nuclear antigen of herpesvirus saimiri are essential for episomal persistence of the viral genome J. Gen. Virol., September 1, 2002; 83(9): 2269 - 2278. [Abstract] [Full Text] [PDF]
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T. Piolot, M. Tramier, M. Coppey, J.-C. Nicolas, and V. Marechal Close but Distinct Regions of Human Herpesvirus 8 Latency-Associated Nuclear Antigen 1 Are Responsible for Nuclear Targeting and Binding to Human Mitotic Chromosomes J. Virol., April 15, 2001; 75(8): 3948 - 3959. [Abstract] [Full Text]
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