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Journal of General Virology (2000), 81, 2665-2673.
© 2000 Society for General Microbiology

Animal: DNA Viruses

The in vivo effects of recombinant bovine herpesvirus-1 expressing bovine interferon-{gamma}

Camilo Raggo1,2, Monique Habermehl2, Lorne A. Babiuk1,2 and Philip Griebel2

Department of Veterinary Microbiology1 and Veterinary Infectious Disease Organization2, 120 Veterinary Road, University of Saskatchewan, Canada S7N 5E3

Author for correspondence: Philip Griebel. Fax +1 306 966 7478. e-mail griebelp{at}sask.usask.ca

To study the biological relevance of using bovine herpesvirus-1 (BHV-1) as a vector for expressing cytokines, a BHV-1 virus that expressed bovine interferon-{gamma} (IFN-{gamma}) was constructed. This recombinant virus (BHV-1/IFN{gamma}) was then used to infect the natural host in a respiratory disease model. In vitro characterization of the recombinant interferon-{gamma} confirmed that the cytokine expressed in BHV-1-infected cells was biologically active. The in vivo effects of the recombinant IFN-{gamma} were then analysed during a primary infection and after reactivation of a latent infection. During the primary infection, similar body temperature, clinical responses and virus shedding were observed for calves infected with either recombinant BHV-1/IFN{gamma} or parental gC-/LacZ+ virus. An analysis of cellular and humoral responses did not reveal any significant immunomodulation by BHV-1/IFN{gamma} during the primary infection. The stability and activity of recombinant IFN-{gamma} was also analysed following the establishment of a latent infection. The presence of recombinant IFN-{gamma} did not significantly alter virus shedding following reactivation. The isolation of reactivated BHV-1/IFN{gamma} virus confirmed that a functional IFN-{gamma} gene was retained during latency. Thus, herpesviruses may provide virus vectors that retain functional genes during latency and recrudescence.




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