| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Animal: RNA Viruses |
Department of Virology and Molecular Biology1 and Department of Immunology2, St Jude Children’s Research Hospital, Memphis, TN 38105-2794, USA
Author for correspondence: Robert Webster. Fax +1 901 523 2622. e-mail robert.webster{at}stjude.org
The continual threat posed by newly emerging influenza virus strains is demonstrated by the recent outbreak of H5N1 influenza virus in Hong Kong. Currently, immunization against influenza virus infection is fairly adequate, but it is imperative that improved vaccines are developed that can protect against a variety of strains and be generated rapidly. Since humoral immunity is ineffective against serologically distinct viruses, one strategy would be to develop vaccines that emphasize cellular immunity. Here we report the successful protection of C57BL/6 mice from a lethal A/HK/156/97 (HK156) infection by immunizing first with an H9N2 isolate, A/Quail/HK/G1/97 (QHKG1), that harbours internal genes 98% homologous to HK156. This strategy also protected mice that are deficient in antibody production, indicating that the immunity is T-cell-mediated. In the course of these studies, we generated a highly pathogenic H5N1 reassortant which implicated NP and PB2 as having an important contribution to pathogenesis when present with a highly cleavable H5. These results provide the first demonstration that protective cell-mediated immunity can be established against the highly virulent HK156 virus and have important implications for the development of novel strategies for the prevention and treatment of HK156 infection and the design of future influenza vaccines.
This article has been cited by other articles:
|
|
 |
|
  J. H. C. M. Kreijtz, G. de Mutsert, C. A. van Baalen, R. A. M. Fouchier, A. D. M. E. Osterhaus, and G. F. Rimmelzwaan Cross-Recognition of Avian H5N1 Influenza Virus by Human Cytotoxic T-Lymphocyte Populations Directed to Human Influenza A Virus J. Virol., June 1, 2008; 82(11): 5161 - 5166. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Webby, S. Andreansky, J. Stambas, J. E. Rehg, R. G. Webster, P. C. Doherty, and S. J. Turner Protection and compensation in the influenza virus-specific CD8+ T cell response PNAS, June 10, 2003; 100(12): 7235 - 7240. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. H. Seo, M. Peiris, and R. G. Webster Protective Cross-Reactive Cellular Immunity to Lethal A/Goose/Guangdong/1/96-Like H5N1 Influenza Virus Is Correlated with the Proportion of Pulmonary CD8+ T Cells Expressing Gamma Interferon J. Virol., April 16, 2002; 76(10): 4886 - 4890. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. A. Benton, J. A. Misplon, C.-Y. Lo, R. R. Brutkiewicz, S. A. Prasad, and S. L. Epstein Heterosubtypic Immunity to Influenza A Virus in Mice Lacking IgA, All Ig, NKT Cells, or {{gamma}}{{delta}} T Cells J. Immunol., June 15, 2001; 166(12): 7437 - 7445. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Lu, M. Renshaw, T. M. Tumpey, G. D. Kelly, J. Hu-Primmer, and J. M. Katz Immunity to Influenza A H9N2 Viruses Induced by Infection and Vaccination J. Virol., May 15, 2001; 75(10): 4896 - 4901. [Abstract] [Full Text]
|
|
|
|
|
|
S. H. Seo and R. G. Webster Cross-Reactive, Cell-Mediated Immunity and Protection of Chickens from Lethal H5N1 Influenza Virus Infection in Hong Kong Poultry Markets J. Virol., March 15, 2001; 75(6): 2516 - 2525. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
