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Journal of General Virology (2000), 81, 2715-2722.
© 2000 Society for General Microbiology

Animal: RNA Viruses

Binding of human respiratory syncytial virus to cells: implication of sulfated cell surface proteoglycans

Isidoro Martínez1 and José A. Melero1

Centro Nacional de Biología Fundamental, Instituto de Salud Carlos III, Majadahonda, 28220 Madrid, Spain1

Author for correspondence: Isidoro Martínez. Fax +34 91 509 7919. e-mail imago{at}isciii.es

Binding of human respiratory syncytial virus (HRSV) to cultured cells was measured by flow cytometry. Using this assay and influenza virus as a control virus with a well-characterized receptor, a systematic search of cell surface molecules that might be implicated in HRSV binding was carried out. Treatment of cells with different enzymes or with other reagents suggested that heparin-like glycosaminoglycans (GAGs) were involved in attachment of HRSV, but not influenza virus, to host cells. This was further confirmed by a lack of binding of HRSV to CHO-K1 mutant cell lines deficient in glycosylation or GAGs biosynthesis and by an inhibition of binding after preincubation of virus with heparin and other GAGs. The degree of sulfation, more than the polysaccharide backbone of GAGs, seems to be critical for virus binding.




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