J Gen Virol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Martínez, I.
Right arrow Articles by Melero, J. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Martínez, I.
Right arrow Articles by Melero, J. A.
Agricola
Right arrow Articles by Martínez, I.
Right arrow Articles by Melero, J. A.
Journal of General Virology (2000), 81, 2715-2722.
© 2000 Society for General Microbiology

Animal: RNA Viruses

Binding of human respiratory syncytial virus to cells: implication of sulfated cell surface proteoglycans

Isidoro Martínez1 and José A. Melero1

Centro Nacional de Biología Fundamental, Instituto de Salud Carlos III, Majadahonda, 28220 Madrid, Spain1

Author for correspondence: Isidoro Martínez. Fax +34 91 509 7919. e-mail imago{at}isciii.es

Binding of human respiratory syncytial virus (HRSV) to cultured cells was measured by flow cytometry. Using this assay and influenza virus as a control virus with a well-characterized receptor, a systematic search of cell surface molecules that might be implicated in HRSV binding was carried out. Treatment of cells with different enzymes or with other reagents suggested that heparin-like glycosaminoglycans (GAGs) were involved in attachment of HRSV, but not influenza virus, to host cells. This was further confirmed by a lack of binding of HRSV to CHO-K1 mutant cell lines deficient in glycosylation or GAGs biosynthesis and by an inhibition of binding after preincubation of virus with heparin and other GAGs. The degree of sulfation, more than the polysaccharide backbone of GAGs, seems to be critical for virus binding.




This article has been cited by other articles:


Home page
 Clin. Microbiol. Rev.Home page
S. S. Mohapatra and S. Boyapalle
Epidemiologic, Experimental, and Clinical Links between Respiratory Syncytial Virus Infection and Asthma
Clin. Microbiol. Rev., July 1, 2008; 21(3): 495 - 504.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
R. L. Crim, S. A. Audet, S. A. Feldman, H. S. Mostowski, and J. A. Beeler
Identification of Linear Heparin-Binding Peptides Derived from Human Respiratory Syncytial Virus Fusion Glycoprotein That Inhibit Infectivity
J. Virol., January 1, 2007; 81(1): 261 - 271.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
K. S. Jones, C. Petrow-Sadowski, D. C. Bertolette, Y. Huang, and F. W. Ruscetti
Heparan Sulfate Proteoglycans Mediate Attachment and Entry of Human T-Cell Leukemia Virus Type 1 Virions into CD4+ T Cells
J. Virol., October 15, 2005; 79(20): 12692 - 12702.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M. Vlasak, I. Goesler, and D. Blaas
Human Rhinovirus Type 89 Variants Use Heparan Sulfate Proteoglycan for Cell Attachment
J. Virol., May 15, 2005; 79(10): 5963 - 5970.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
H. San-Juan-Vergara, M. E. Peeples, R. F. Lockey, and S. S. Mohapatra
Protein Kinase C-{alpha} Activity Is Required for Respiratory Syncytial Virus Fusion to Human Bronchial Epithelial Cells
J. Virol., December 15, 2004; 78(24): 13717 - 13726.
[Abstract] [Full Text] [PDF]

Home page
 J Antimicrob ChemotherHome page
P. J. Budge and B. S. Graham
Inhibition of respiratory syncytial virus by RhoA-derived peptides: implications for the development of improved antiviral agents targeting heparin-binding viruses
J. Antimicrob. Chemother., August 1, 2004; 54(2): 299 - 302.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
P. J. Budge, Y. Li, J. A. Beeler, and B. S. Graham
RhoA-Derived Peptide Dimers Share Mechanistic Properties with Other Polyanionic Inhibitors of Respiratory Syncytial Virus (RSV), Including Disruption of Viral Attachment and Dependence on RSV G
J. Virol., May 15, 2004; 78(10): 5015 - 5022.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
E. Escribano-Romero, J. Rawling, B. Garcia-Barreno, and J. A. Melero
The Soluble Form of Human Respiratory Syncytial Virus Attachment Protein Differs from the Membrane-Bound Form in Its Oligomeric State but Is Still Capable of Binding to Cell Surface Proteoglycans
J. Virol., April 1, 2004; 78(7): 3524 - 3532.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J. L. Douglas, M. L. Panis, E. Ho, K.-Y. Lin, S. H. Krawczyk, D. M. Grant, R. Cai, S. Swaminathan, and T. Cihlar
Inhibition of Respiratory Syncytial Virus Fusion by the Small Molecule VP-14637 via Specific Interactions with F Protein
J. Virol., May 1, 2003; 77(9): 5054 - 5064.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
J. Schlender, G. Zimmer, G. Herrler, and K.-K. Conzelmann
Respiratory Syncytial Virus (RSV) Fusion Protein Subunit F2, Not Attachment Protein G, Determines the Specificity of RSV Infection
J. Virol., April 15, 2003; 77(8): 4609 - 4616.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
A. G. P. Oomens, A. G. Megaw, and G. W. Wertz
Infectivity of a Human Respiratory Syncytial Virus Lacking the SH, G, and F Proteins Is Efficiently Mediated by the Vesicular Stomatitis Virus G Protein
J. Virol., March 15, 2003; 77(6): 3785 - 3798.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
M. Iqbal and J. W. McCauley
Identification of the glycosaminoglycan-binding site on the glycoprotein Erns of bovine viral diarrhoea virus by site-directed mutagenesis
J. Gen. Virol., September 1, 2002; 83(9): 2153 - 2159.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
G. Zimmer, K.-K. Conzelmann, and G. Herrler
Cleavage at the Furin Consensus Sequence RAR/KR109 and Presence of the Intervening Peptide of the Respiratory Syncytial Virus Fusion Protein Are Dispensable for Virus Replication in Cell Culture
J. Virol., August 12, 2002; 76(18): 9218 - 9224.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
L. Zhang, M. E. Peeples, R. C. Boucher, P. L. Collins, and R. J. Pickles
Respiratory Syncytial Virus Infection of Human Airway Epithelial Cells Is Polarized, Specific to Ciliated Cells, and without Obvious Cytopathology
J. Virol., May 3, 2002; 76(11): 5654 - 5666.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. J. Gorman, J. L. McKimm-Breschkin, R. S. Norton, and K. J. Barnham
Antiviral Activity and Structural Characteristics of the Nonglycosylated Central Subdomain of Human Respiratory Syncytial Virus Attachment (G) Glycoprotein
J. Biol. Chem., October 12, 2001; 276(42): 38988 - 38994.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M. C. Dechecchi, P. Melotti, A. Bonizzato, M. Santacatterina, M. Chilosi, and G. Cabrini
Heparan Sulfate Glycosaminoglycans Are Receptors Sufficient To Mediate the Initial Binding of Adenovirus Types 2 and 5
J. Virol., September 15, 2001; 75(18): 8772 - 8780.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
G. Zimmer, I. Trotz, and G. Herrler
N-Glycans of F Protein Differentially Affect Fusion Activity of Human Respiratory Syncytial Virus
J. Virol., May 15, 2001; 75(10): 4744 - 4751.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
G. Zimmer, L. Budz, and G. Herrler
Proteolytic Activation of Respiratory Syncytial Virus Fusion Protein. CLEAVAGE AT TWO FURIN CONSENSUS SEQUENCES
J. Biol. Chem., August 17, 2001; 276(34): 31642 - 31650.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2000 by the Society for General Microbiology.