| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Animal: RNA Viruses |
Departamento de Genética y Fisiología Molecular, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apartado Postal 510-3, Colonia Miraval, Cuernavaca, Morelos 62250, Mexico1
Author for correspondence: Ernesto Méndez. Fax +52 73 17 2388. e-mail ernesto{at}ibt.unam.mx
Human astroviruses are an important cause of gastroenteritis. As part of a molecular epidemiological study carried out in Mexico a human astrovirus isolate, Yuc-8, was adapted to grow in CaCo-2 cells, and its entire genome was sequenced. A 15 amino acid deletion in ORF1a, which has been associated with adaptation of astroviruses to grow in cells other than CaCo-2, was present in Yuc-8. Comparative sequence analysis of the Yuc-8 ORF2 with reported human astrovirus sequences revealed that this isolate belongs to genotype (serotype) 8. Two distinct domains in ORF2 were observed: an amino-terminal domain (residues 1 to 415), with identities higher than 81% among the strains analysed, and a carboxy-terminal domain (residues 416 to 782) with identities between 36 and 60%. Two non-superimposable phylogenetic trees were generated by separate analysis of these two domains, suggesting that a differential selective pressure is exerted along the structural polyprotein.
This article has been cited by other articles:
|
|
 |
|
  Y. Fu, M. Pan, X. Wang, Y. Xu, X. Xie, N. J. Knowles, H. Yang, and D. Zhang Complete sequence of a duck astrovirus associated with fatal hepatitis in ducklings J. Gen. Virol., May 1, 2009; 90(5): 1104 - 1108. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Mendez, G. Aguirre-Crespo, G. Zavala, and C. F. Arias Association of the Astrovirus Structural Protein VP90 with Membranes Plays a Role in Virus Morphogenesis J. Virol., October 1, 2007; 81(19): 10649 - 10658. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Guix, S. Caballero, A. Bosch, and R. M. Pinto C-Terminal nsP1a Protein of Human Astrovirus Colocalizes with the Endoplasmic Reticulum and Viral RNA J. Virol., December 15, 2004; 78(24): 13627 - 13636. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Mendez, E. Salas-Ocampo, and C. F. Arias Caspases Mediate Processing of the Capsid Precursor and Cell Release of Human Astroviruses J. Virol., August 15, 2004; 78(16): 8601 - 8608. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Mendez-Toss, D. D. Griffin, J. Calva, J. F. Contreras, F. I. Puerto, F. Mota, H. Guiscafre, R. Cedillo, O. Munoz, I. Herrera, et al. Prevalence and Genetic Diversity of Human Astroviruses in Mexican Children with Symptomatic and Asymptomatic Infections J. Clin. Microbiol., January 1, 2004; 42(1): 151 - 157. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Mittelholzer, K.-O. Hedlund, L. Englund, H.-H. Dietz, and L. Svensson Molecular characterization of a novel astrovirus associated with disease in mink J. Gen. Virol., November 1, 2003; 84(11): 3087 - 3094. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Mendez, M. P. E. Salas-Ocampo, M. E. Munguia, and C. F. Arias Protein Products of the Open Reading Frames Encoding Nonstructural Proteins of Human Astrovirus Serotype 8 J. Virol., November 1, 2003; 77(21): 11378 - 11384. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Nadan, J. E. Walter, W. O. K. Grabow, D. K. Mitchell, and M. B. Taylor Molecular Characterization of Astroviruses by Reverse Transcriptase PCR and Sequence Analysis: Comparison of Clinical and Environmental Isolates from South Africa Appl. Envir. Microbiol., February 1, 2003; 69(2): 747 - 753. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Chikhi-Brachet, F. Bon, L. Toubiana, P. Pothier, J.-C. Nicolas, A. Flahault, and E. Kohli Virus Diversity in a Winter Epidemic of Acute Diarrhea in France J. Clin. Microbiol., November 1, 2002; 40(11): 4266 - 4272. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Mendez, T. Fernandez-Luna, S. Lopez, M. Mendez-Toss, and C. F. Arias Proteolytic Processing of a Serotype 8 Human Astrovirus ORF2 Polyprotein J. Virol., July 17, 2002; 76(16): 7996 - 8002. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. V. Lukashov and J. Goudsmit Evolutionary relationships among Astroviridae J. Gen. Virol., June 1, 2002; 83(6): 1397 - 1405. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Geigenmuller, N. H. Ginzton, and S. M. Matsui Studies on intracellular processing of the capsid protein of human astrovirus serotype 1 in infected cells J. Gen. Virol., June 1, 2002; 83(7): 1691 - 1695. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Geigenmuller, T. Chew, N. Ginzton, and S. M. Matsui Processing of Nonstructural Protein 1a of Human Astrovirus J. Virol., February 15, 2002; 76(4): 2003 - 2008. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Kiang and S. M. Matsui Proteolytic processing of a human astrovirus nonstructural protein J. Gen. Virol., January 1, 2002; 83(1): 25 - 34. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Guix, S. Caballero, C. Villena, R. Bartolome, C. Latorre, N. Rabella, M. Simo, A. Bosch, and R. M. Pinto Molecular Epidemiology of Astrovirus Infection in Barcelona, Spain J. Clin. Microbiol., January 1, 2002; 40(1): 133 - 139. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
