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Animal: RNA Viruses |
Institut für Virologie und Immunbiologie, Universität Würzburg, Germany1
Institut für Virologie im MTZ, Medizinische Fakultät ‘Carl Gustav Carus’, Technische Universität Dresden, Fiedlerstr. 42, 01307 Dresden, Germany2
Author for correspondence: Axel Rethwilm (at the Technische Universität Dresden). Fax +49 351 458 6314. e-mail Axel.Rethwilm{at}mailbox.tu-dresden.de
Mouse monoclonal antibodies (MAbs) that specifically detect the 127 kDa Pol precursor and the 85 kDa reverse transcriptase/RNase H (RT/RN) or pr127 and the 40 kDa integrase (IN) in immunoblot and immunofluorescence assays (IFA) were used to investigate the subcellular localization of primate foamy virus (PFV) proteins. IFA of cells infected with PFV using the anti-Pol MAbs and rabbit anti-capsid (Gag) serum revealed that both the Gag and Pol proteins are transported into the nucleus. Transfection of cells with eukaryotic expression constructs for pr127Pol, p85RT/RN and p40IN served to show Gag-independent subcellular localization of Pol proteins. Interestingly, not only the Pol precursor and IN molecules were found to be localized to the nucleus, but also the RT/RN subdomain. It is therefore suggested that PFV cores bear at least three separate nuclear localization signals, one in Gag and two in Pol. The latter appear to be localized to the two Pol subdomains.
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