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Animal: DNA Viruses |
Department of Molecular & Medical Pharmacology, UCLA AIDS Institute, Jonsson Comprehensive Cancer Center, and the Molecular Biology Institute, University of California at Los Angeles, Los Angeles, CA 90095, USA1
Author for correspondence: Ren Sun (at Department of Molecular & Medical Pharmacology). Fax +1 310 825 6267. e-mail rsun{at}mednet.ucla.edu
Rta, mainly encoded by open reading frame 50 (ORF50), is the product of an immediate-early gene of human herpesvirus-8 (HHV-8)/Kaposi’s sarcoma-associated herpesvirus. Rta is a transcriptional activator that is both necessary and sufficient to disrupt viral latency and activate the expression of downstream viral lytic genes. We report that ectopically expressed Rta protein could also activate the rta promoter on a reporter plasmid up to 144-fold, both in latently infected B cells and in uninfected epithelial cells, and that this activation was dose-dependent. Furthermore, by analysing the 5' untranslated region using ribonuclease protection assays, we demonstrated that transfection of an Rta expression plasmid into latently infected cells activated the expression of rta transcripts from endogenous viral genomes. We propose that auto-activation of the immediate-early gene, rta, is an important strategy for HHV-8 to effectively respond to environmental stimuli and maximally activate the virus lytic cycle.
This article has been cited by other articles:
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T.-T. Wu, L. Tong, T. Rickabaugh, S. Speck, and R. Sun Function of Rta Is Essential for Lytic Replication of Murine Gammaherpesvirus 68 J. Virol., October 1, 2001; 75(19): 9262 - 9273. [Abstract] [Full Text] [PDF]
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