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Insect |
Department of Entomology, University of Wisconsin-Madison, Madison, WI 53706, USA1
Author for correspondence: Mike Strand. Fax +1 608 262 3322. e-mail mrstrand{at}facstaff.wisc.edu
The braconid wasp Microplitis demolitor carries M. demolitor polydnavirus (MdPDV) and parasitizes the larval stage of the moth Pseudoplusia includens. M. demolitor injects MdPDV into P. includens larvae when it lays an egg and the virus infects various cells including haemocytes. Two new MdPDV transcripts expressed in host haemocytes were characterized in this study. Screening of an MdPDV-infected haemocyte cDNA library identified a 0·4 kb cDNA encoding a predicted protein of 103 amino acids which was named Egf0·4. This protein contained a cysteine-rich epidermal growth factor (EGF)-like motif at its N terminus that was similar to the EGF-like domains in the previously identified MdPDV genes egf1·5 and egf1·0. Sequencing of the genomic clone pMd-10 indicated that it contained the egf0·4 gene, which consisted of two introns and three exons. This gene was located on MdPDV segment O and appeared to exist in multiple copies. A nucleic acid and expression screen identified a 1·8 kb cDNA encoding a predicted protein of 515 amino acids designated Glc1·8. This protein consisted of a heavily glycosylated central core of six tandemly arranged repeats flanked by hydrophobic N- and C-terminal domains. Northern blotting and in situ hybridization studies indicated that both egf0·4 and glc1·8 were expressed in MdPDV-infected host haemocytes. Immunocytochemical studies also indicated that Glc1·8 localized to the cell surface.
This article has been cited by other articles:
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  Z. Lu, M. H. Beck, Y. Wang, H. Jiang, and M. R. Strand The Viral Protein Egf1.0 Is a Dual Activity Inhibitor of Prophenoloxidase-activating Proteinases 1 and 3 from Manduca sexta J. Biol. Chem., August 1, 2008; 283(31): 21325 - 21333. [Abstract] [Full Text] [PDF]
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 M. H. Beck and M. R. Strand A novel polydnavirus protein inhibits the insect prophenoloxidase activation pathway PNAS, December 4, 2007; 104(49): 19267 - 19272. [Abstract] [Full Text] [PDF]
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 A. J. Pruijssers and M. R. Strand PTP-H2 and PTP-H3 from Microplitis demolitor Bracovirus Localize to Focal Adhesions and Are Antiphagocytic in Insect Immune Cells J. Virol., February 1, 2007; 81(3): 1209 - 1219. [Abstract] [Full Text] [PDF]
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H. Thoetkiattikul, M. H. Beck, and M. R. Strand Inhibitor {kappa}B-like proteins from a polydnavirus inhibit NF-{kappa}B activation and suppress the insect immune response PNAS, August 9, 2005; 102(32): 11426 - 11431. [Abstract] [Full Text] [PDF]
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M. Beck and M. R. Strand Glc1.8 from Microplitis demolitor Bracovirus Induces a Loss of Adhesion and Phagocytosis in Insect High Five and S2 Cells J. Virol., February 1, 2005; 79(3): 1861 - 1870. [Abstract] [Full Text] [PDF]
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