J Gen Virol Try IJSEM Online
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Corrigendum (v81,p1629)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sibbet, G.
Right arrow Articles by Campo, M. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sibbet, G.
Right arrow Articles by Campo, M. S.
Agricola
Right arrow Articles by Sibbet, G.
Right arrow Articles by Campo, M. S.
Journal of General Virology (2000), 81, 327-334.
© 2000 Society for General Microbiology

Animal: DNA Viruses

{alpha}6 integrin is not the obligatory cell receptor for bovine papillomavirus type 4

Gary Sibbet1, Christine Romero-Graillet2, Guerrino Meneguzzi2 and M. Saveria Campob,1

Beatson Institute for Cancer Research, Garscube Estate, Glasgow G61 1QH, UK1
INSERM U385, Faculty of Medicine, University of Nice, Nice, France2

Author for correspondence: Gary Sibbet. Fax +44 141 942 6521. e-mail g.sibbet{at}beatson.gla.ac.uk

Recently, {alpha}6 integrin has been proposed as the epithelial cell receptor for papillomavirus. This study investigated whether {alpha}6 integrin is the cellular receptor for bovine papillomavirus type 4 (BPV-4), which is strictly epitheliotropic and infects the mucous epithelium of the upper digestive tract. Primary bovine mucosal keratinocytes from the palate of a foetus (PalK) displayed high levels of {alpha}6 integrin; matched primary fibroblasts from the same biopsy (PalF) expressed almost no {alpha}6 integrin. However, BPV-4 bound both PalK and PalF to similar, saturable levels. Native BPV-4 virions infected PalK in vitro, as detected by RT–PCR of E7 RNA. Infection could be blocked by excess virus-like particles (VLPs) and by neutralizing antisera against L1–L2 and L1 VLPs or by denaturation of the virions, supporting the view that infection in vitro mimics the process in vivo. {alpha}6 integrin-negative human keratinocyte cell lines were derived from patients affected by junctional epidermolysis bullosa presenting genetic lesions in their hemidesmosomes. The level of {alpha}6 integrin expression was determined in these cell lines by in situ immunofluorescence and FACS. Despite the absence of {alpha}6 integrin expression by BO-SV cells, they were bound by BPV-4 to similar, saturable levels as normal keratinocytes, KH-SV. Furthermore, BO-SV and KH-SV cells were both infected by BPV-4 to apparently the same extent as PalK cells. These results are consistent with the conclusion that {alpha}6 integrin is not the obligatory receptor for a bovine mucosotropic papillomavirus.




This article has been cited by other articles:


Home page
 J. Virol.Home page
T. D. Culp, L. R. Budgeon, M. P. Marinkovich, G. Meneguzzi, and N. D. Christensen
Keratinocyte-secreted laminin 5 can function as a transient receptor for human papillomaviruses by binding virions and transferring them to adjacent cells.
J. Virol., September 1, 2006; 80(18): 8940 - 8950.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
N. A. Patterson, J. L. Smith, and M. A. Ozbun
Human Papillomavirus Type 31b Infection of Human Keratinocytes Does Not Require Heparan Sulfate
J. Virol., June 1, 2005; 79(11): 6838 - 6847.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
R. Yang, P. M. Day, W. H. Yutzy IV, K.-Y. Lin, C.-F. Hung, and R. B. S. Roden
Cell Surface-Binding Motifs of L2 That Facilitate Papillomavirus Infection
J. Virol., March 15, 2003; 77(6): 3531 - 3541.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
K.-N. Zhao and I. H. Frazer
Saccharomyces cerevisiae Is Permissive for Replication of Bovine Papillomavirus Type 1
J. Virol., October 25, 2002; 76(23): 12265 - 12273.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
N. D. Christensen, C. A. Reed, T. D. Culp, P. L. Hermonat, M. K. Howett, R. A. Anderson, and L. J. D. Zaneveld
Papillomavirus Microbicidal Activities of High-Molecular-Weight Cellulose Sulfate, Dextran Sulfate, and Polystyrene Sulfonate
Antimicrob. Agents Chemother., December 1, 2001; 45(12): 3427 - 3432.
[Abstract] [Full Text] [PDF]

Home page
 Int ImmunolHome page
D. M. Da Silva, M. P. Velders, J. D. Nieland, J. T. Schiller, B. J. Nickoloff, and W. M. Kast
Physical interaction of human papillomavirus virus-like particles with immune cells
Int. Immunol., May 1, 2001; 13(5): 633 - 641.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
Y. Kawana, K. Kawana, H. Yoshikawa, Y. Taketani, K. Yoshiike, and T. Kanda
Human Papillomavirus Type 16 Minor Capsid Protein L2 N-Terminal Region Containing a Common Neutralization Epitope Binds to the Cell Surface and Enters the Cytoplasm
J. Virol., March 1, 2001; 75(5): 2331 - 2336.
[Abstract] [Full Text]

Home page
 J. Virol.Home page
T. Giroglou, L. Florin, F. Schäfer, R. E. Streeck, and M. Sapp
Human Papillomavirus Infection Requires Cell Surface Heparan Sulfate
J. Virol., February 1, 2001; 75(3): 1565 - 1570.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2000 by the Society for General Microbiology.