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Replication of Aleutian mink disease parvovirus in mink lymph node histocultures

Laboratory of Persistent Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, MT 59840, USA¹
Laboratory of Virology and Immunology, Institute of Veterinary Microbiology, The Royal Veterinary and Agricultural University, Ridebane 3, DK-1870 Frederiksberg C, Denmark²

Author for correspondence: Marshall E. Bloom. Fax +1 406 363 9286. e-mail mbloom{at}niaid.nih.gov

Aleutian mink disease parvovirus (ADV), causes an immune disorder with a persistent infection of lymphoid organs in adult mink. We studied replication of ADV in gel-supported histocultures prepared from adult mink mesenteric lymph node (MLN). Evidence of virus replication in the histocultures was first observed by indirect immunofluorescence 72 h after incubation with virus. Cells resembling lymphocytes and macrophages contained both ADV capsid (VP2) and nonstructural (NS1 and NS2) proteins, and were present in a distribution suggestive of infected cells within germinal centres. ADV replicative form and encapsidated virion DNA were also detected in infected histocultures at time-points after 72 h. In addition, we were able to passage ADV-Utah to a new round of histocultures. These results suggested that the infected cells were actual target cells for ADV replication and that productive ADV-Utah replication, complete with the generation of virus, was occurring in the histocultures. The mink MLN histocultures provide a system to study the replication and molecular pathogenesis of ADV in target tissues.

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				S. M. Best, J. F. Shelton, J. M. Pompey, J. B. Wolfinbarger, and M. E. Bloom Caspase Cleavage of the Nonstructural Protein NS1 Mediates Replication of Aleutian Mink Disease Parvovirus J. Virol., May 1, 2003; 77(9): 5305 - 5312. [Abstract] [Full Text] [PDF]