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Animal: RNA Viruses |
Department of Human Retrovirology, Academic Medical Centre, University of Amsterdam, Meibergdreef 45, 1105BA Amsterdam, The Netherlands1
Author for correspondence: Marion Cornelissen. Fax +31 20 5669062. e-mail M.I.Cornelissen{at}amc.uva.nl
To identify new subtype G human immunodeficiency virus type 1 (HIV-1) strains and AG recombinant forms, we collected 28 serum samples from immigrants to the Netherlands from 12 countries throughout Africa. Based on the gag sequences 22 isolates were identified as subtype A or G. Phylogenetic analysis of discontinuous regions of the gag (726 nt), pol (1176 nt) and env (276 nt) genes revealed 13 AG recombinants with the mosaic structure Agag/Gpol/Aenv, three with Agag/Gpol/Genv and one other with Agag /Gpol/Genv , in addition to ‘pure’ subtypes Agag/Apol/Aenv (n=1) and Ggag/Gpol/Genv (n=4). To analyse the crossover points in more detail, a new RT–PCR was developed resulting in a large contiguous sequence of 2600 nt from the gag region to half the pol region. All the 13 Agag/Gpol/Aenv recombinants appeared to belong to the circulating recombinant form (CRF) AG (IbNG). The three Agag/Gpol /Genv recombinants differed from the CRF AG (IbNG) subtype, suggesting the identification of a new CRF subtype. The recovery of AG recombinants from African countries a thousand miles apart indicates the active spread of new recombinants.
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