HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Wintermantel, W. M. Articles by Zaitlin, M. Search for Related Content PubMed PubMed Citation Articles by Wintermantel, W. M. Articles by Zaitlin, M. Agricola Articles by Wintermantel, W. M. Articles by Zaitlin, M.

Transgene translatability increases effectiveness of replicase-mediated resistance to Cucumber mosaic virus

Department of Plant Pathology, Cornell University, Ithaca, NY 14853, USA¹

Author for correspondence: William Wintermantel. Present address: USDA-ARS, 1636 E. Alisal Street, Salinas, CA 93905, USA. Fax +1 831 755 2814. e-mail bwinter{at}pwa.ars.usda.gov

Transgenic tobacco plants expressing an altered form of the 2a replicase gene from the Fny strain of Cucumber mosaic virus (CMV) exhibit suppressed virus replication and restricted virus movement when inoculated mechanically or by aphid vectors. Additional transformants have been generated which contain replicase gene constructs designed to determine the role(s) of transgene mRNA and/or protein in resistance. Resistance to systemic disease caused by CMV, as well as delayed infection, was observed in several lines of transgenic plants which were capable of expressing either full-length or truncated replicase proteins. In contrast, among plants which contained nontranslatable transgene constructs, only one of 61 lines examined exhibited delays or resistance. Once infected, plants never recovered, regardless of transgene translatability. Transgenic plants exhibiting a range of resistance levels were examined for transgene copy number, mRNA and protein levels. Although ribonuclease protection assays demonstrated that transgene mRNA levels were very low, resistant lines had consistently more steady-state transgene mRNA than susceptible lines. Furthermore, chlorotic or necrotic local lesions developed on the inoculated leaves of transgenic lines containing translatable transgenes, but not on inoculated leaves of lines containing nontranslatable transgenes. These results demonstrate that translatability of the transgene and possibly expression of the transgene protein itself facilitates replicase-mediated resistance to CMV in tobacco.

This article has been cited by other articles:

				H. Ziebell, T. Payne, J. O. Berry, J. A. Walsh, and J. P. Carr A cucumber mosaic virus mutant lacking the 2b counter-defence protein gene provides protection against wild-type strains J. Gen. Virol., October 1, 2007; 88(10): 2862 - 2871. [Abstract] [Full Text] [PDF]

				T. Canto and P. Palukaitis A Cucumber Mosaic Virus (CMV) RNA 1 Transgene Mediates Suppression of the Homologous Viral RNA 1 Constitutively and Prevents CMV Entry into the Phloem J. Virol., October 1, 2001; 75(19): 9114 - 9120. [Abstract] [Full Text] [PDF]