| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Animal: RNA Viruses |
Division of Molecular Biology, Institute for Animal Health, Compton Laboratory, Compton, Newbury, Berkshire RG20 7NN, UK1
Author for correspondence: Paul Britton. Fax +44 1635 577263. e-mail paul.britton{at}bbsrc.ac.uk
A defective RNA (D-RNA), CD-61, derived from the Beaudette strain of the avian coronavirus infectious bronchitis virus (IBV), was rescued (replicated and packaged) using four heterologous strains of IBV as helper virus. Sequence analysis of the genomic RNA from the four heterologous IBV strains (M41, H120, HV10 and D207) identified nucleotide differences of up to 17% within the leader sequence and up to 4·3% within the whole of the adjacent 5' untranslated region (UTR). Analysis of the 5' ends of the rescued D-RNAs showed that the Beaudette leader sequence, present on the initial CD-61, had been replaced with the corresponding leader sequence from the helper IBV strain but the adjacent 5' UTR sequence of the rescued D-RNAs corresponded to the original CD-61 Beaudette sequence. These results demonstrated that the phenomenon of leader switching previously identified for the coronaviruses murine hepatitis virus and bovine coronavirus (BCoV) also occurred during the replication of IBV D-RNAs. Three predicted stem–loop structures were identified within the 5' UTR of IBV. Stem–loop I showed a high degree of covariance amongst the IBV strains providing phylogenetic evidence that this structure exists and is potentially involved in replication, supporting previous observations that a BCoV stem–loop homologue was essential for replication of BCoV defective interfering RNAs.
This article has been cited by other articles:
|
|
 |
|
  A. O. Pasternak, W. J. M. Spaan, and E. J. Snijder Nidovirus transcription: how to make sense...? J. Gen. Virol., June 1, 2006; 87(6): 1403 - 1421. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Casais, M. Davies, D. Cavanagh, and P. Britton Gene 5 of the Avian Coronavirus Infectious Bronchitis Virus Is Not Essential for Replication J. Virol., July 1, 2005; 79(13): 8065 - 8078. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Chen, A. Gill, B. K. Dove, S. R. Emmett, C. F. Kemp, M. A. Ritchie, M. Dee, and J. A. Hiscox Mass Spectroscopic Characterization of the Coronavirus Infectious Bronchitis Virus Nucleoprotein and Elucidation of the Role of Phosphorylation in RNA Binding by Using Surface Plasmon Resonance J. Virol., January 15, 2005; 79(2): 1164 - 1179. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Dove, D. Cavanagh, and P. Britton Presence of an Encephalomyocarditis Virus Internal Ribosome Entry Site Sequence in Avian Infectious Bronchitis Virus Defective RNAs Abolishes Rescue by Helper Virus J. Virol., March 15, 2004; 78(6): 2711 - 2721. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Hackney, D. Cavanagh, P. Kaiser, and P. Britton In Vitro and In Ovo Expression of Chicken Gamma Interferon by a Defective RNA of Avian Coronavirus Infectious Bronchitis Virus J. Virol., May 15, 2003; 77(10): 5694 - 5702. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Casais, V. Thiel, S. G. Siddell, D. Cavanagh, and P. Britton Reverse Genetics System for the Avian Coronavirus Infectious Bronchitis Virus J. Virol., December 15, 2001; 75(24): 12359 - 12369. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Dalton, R. Casais, K. Shaw, K. Stirrups, S. Evans, P. Britton, T. D. K. Brown, and D. Cavanagh cis-Acting Sequences Required for Coronavirus Infectious Bronchitis Virus Defective-RNA Replication and Packaging J. Virol., January 1, 2001; 75(1): 125 - 133. [Abstract] [Full Text]
|
|
|
|
|
|
S. Evans, D. Cavanagh, and P. Britton Utilizing fowlpox virus recombinants to generate defective RNAs of the coronavirus infectious bronchitis virus J. Gen. Virol., December 1, 2000; 81(12): 2855 - 2865. [Abstract] [Full Text]
|
|
|
|
|
|
K. Stirrups, K. Shaw, S. Evans, K. Dalton, R. Casais, D. Cavanagh, and P. Britton Expression of reporter genes from the defective RNA CD-61 of the coronavirus infectious bronchitis virus J. Gen. Virol., July 1, 2000; 81(7): 1687 - 1698. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
