J Gen Virol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Teycheney, P.-Y.
Right arrow Articles by Tepfer, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Teycheney, P.-Y.
Right arrow Articles by Tepfer, M.
Agricola
Right arrow Articles by Teycheney, P.-Y.
Right arrow Articles by Tepfer, M.
Journal of General Virology (2000), 81, 1121-1126.
© 2000 Society for General Microbiology

Plant

Synthesis of (-)-strand RNA from the 3' untranslated region of plant viral genomes expressed in transgenic plants upon infection with related viruses

Pierre-Yves Teycheney1, Rachid Aaziz1, Sylvie Dinant1, Katalin Salánki2, Colette Tourneur1, Ervin Balázs2, Mireille Jacquemond3 and Mark Tepfer1

INRA, Laboratoire de biologie cellulaire, F-78026 Versailles cedex, France1
Agricultural Biotechnology Center, POB 411, H-2100 Gödöllö, Hungary2
INRA, Domaine Saint Maurice, Station de pathologie végétale, F-84143 Montfavet cedex, France3

Author for correspondence: Pierre-Yves Teycheney. Fax +33 1 30 83 30 99. e-mail teychene{at}versailles.inra.fr

When expressed in transgenic tobacco plants, transgene mRNA that includes the 3' untranslated region (3' UTR) of Lettuce mosaic virus served as template for synthesis of complementary (-)-strand RNA following an infection by Tobacco etch virus, Tobacco vein mottle virus or Pepper mottle virus, but not when infected with Cucumber mosaic virus. Deletion of the 3' UTR from the transgene abolished the synthesis of (-)-strand transcripts. Similar results were obtained in transgenic tobacco plants expressing mRNA that includes the RNA3 3' UTR of Cucumber mosaic virus when infected with Tomato aspermy virus. These results show that the viral RNA-dependent RNA polymerase of several potyviruses and Tomato aspermy virus have the ability to recognize heterologous 3' UTRs when included in transgene mRNAs, and to use them as transcription promoters.




This article has been cited by other articles:


Home page
 J. Gen. Virol.Home page
C. Turturo, A. Friscina, S. Gaubert, M. Jacquemond, J. R. Thompson, and M. Tepfer
Evaluation of potential risks associated with recombination in transgenic plants expressing viral sequences
J. Gen. Virol., January 1, 2008; 89(1): 327 - 335.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
C. Spetz, A. M. Taboada, S. Darwich, J. Ramsell, L. F. Salazar, and J. P. T. Valkonen
Molecular resolution of a complex of potyviruses infecting solanaceous crops at the centre of origin in Peru
J. Gen. Virol., September 1, 2003; 84(9): 2565 - 2578.
[Abstract] [Full Text] [PDF]

Home page
 J. Gen. Virol.Home page
F. Aparicio, J. A. Sánchez-Navarro, R. C. L. Olsthoorn, V. Pallás, and J. F. Bol
Recognition of cis-acting sequences in RNA 3 of Prunus necrotic ringspot virus by the replicase of Alfalfa mosaic virus
J. Gen. Virol., April 1, 2001; 82(4): 947 - 951.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 2000 by the Society for General Microbiology.