HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Lockhart, B. E. Articles by Olszewski, N. E. Search for Related Content PubMed PubMed Citation Articles by Lockhart, B. E. Articles by Olszewski, N. E. Agricola Articles by Lockhart, B. E. Articles by Olszewski, N. E.

Characterization and genomic analysis of tobacco vein clearing virus, a plant pararetrovirus that is transmitted vertically and related to sequences integrated in the host genome

Departments of Plant Pathology and ²Plant Biology, University of Minnesota, St Paul, MN 55108, USA

Author for correspondence: Ben Lockhart. Fax +1 612 625 9728. e-mail plpa{at}puccini.crl.umn.edu

A previously undescribed caulimo-like virus was identified in the hybrid tobacco species Nicotiana edwardsonii, and was named tobacco vein clearing virus (TVCV) after the symptoms associated with its occurrence in this plant. The virions of TVCV are 50 nm in diameter and are composed of a 45 kDa capsid protein and a 7767 bp dsDNA genome. Each strand of the genome is interrupted by a site-specific discontinuity. In genome sequence and arrangement of ORFs TVCV was most similar to cassava vein mosaic virus, indicating that TVCV is a pararetrovirus. No serological relationship was detected between TVCV and any other caulimoviruses, including petunia vein clearing virus, which has similar biological properties. In N. edwardsonii TVCV was seed-transmitted to 100% of progeny plants, but was not transmitted by mechanical inoculation, grafting or Myzus persicae to any of seven other Nicotiana spp. Genomic DNA of TVCV hybridized to genomic DNA of N. edwardsonii and of N. glutinosa, its male parent, but not to genomic DNA of N. clevelandii, the female parent. TVCV has 78% sequence identity with pararetrovirus-like sequences that are present in high copy number in the N. tabacum genome, and TVCV genomic DNA hybridized to genomic DNA of N. tabacum and N. rustica. These observations suggest that the episomal form of TVCV may arise from integrated pararetroviral elements present in N. edwardsonii, that these integrants were inherited from the male parent N. glutinosa, and that these elements are related but not identical to pararetroviral elements occurring in other Nicotiana spp.

This article has been cited by other articles:

				P. Gayral, J.-C. Noa-Carrazana, M. Lescot, F. Lheureux, B. E. L. Lockhart, T. Matsumoto, P. Piffanelli, and M.-L. Iskra-Caruana A Single Banana Streak Virus Integration Event in the Banana Genome as the Origin of Infectious Endogenous Pararetrovirus J. Virol., July 1, 2008; 82(13): 6697 - 6710. [Abstract] [Full Text] [PDF]

				M. Shababi, J. Bourque, K. Palanichelvam, A. Cole, D. Xu, X.-F. Wan, and J. Schoelz The ribosomal shunt translation strategy of cauliflower mosaic virus has evolved from ancient long terminal repeats. J. Virol., April 1, 2006; 80(8): 3811 - 3822. [Abstract] [Full Text] [PDF]

				A. D. W. Geering, N. E. Olszewski, G. Harper, B. E. L. Lockhart, R. Hull, and J. E. Thomas Banana contains a diverse array of endogenous badnaviruses J. Gen. Virol., February 1, 2005; 86(2): 511 - 520. [Abstract] [Full Text] [PDF]

				W. Gregor, M. F. Mette, C. Staginnus, M. A. Matzke, and A. J.M. Matzke A Distinct Endogenous Pararetrovirus Family in Nicotiana tomentosiformis, a Diploid Progenitor of Polyploid Tobacco Plant Physiology, March 1, 2004; 134(3): 1191 - 1199. [Abstract] [Full Text] [PDF]