HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Segouffin-Cariou, C. Articles by Gruffat, H. Search for Related Content PubMed PubMed Citation Articles by Segouffin-Cariou, C. Articles by Gruffat, H. Agricola Articles by Segouffin-Cariou, C. Articles by Gruffat, H.

Characterization of the Epstein–Barr virus BRRF1 gene, located between early genes BZLF1 and BRLF1

Unité de Virologie Humaine U412 INSERM, Ecole Normale Supérieure de Lyon, 46 Allée d’Italie, 69364 Lyon cedex 07, France¹

Author for correspondence: Alain Sergeant. Fax +33 72 72 87 77. e-mail alain.sergeant{at}cri.ens-lyon.fr

The switch from latency to a productive cycle in Epstein–Barr virus (EBV)-infected B cells proliferating in vitro is thought to be due to the transcriptional activation of two viral genes, BZLF1 and BRLF1, encoding two transcription factors called EB1 and R respectively. However, a third gene, BRRF1 is contained in the BZLF1/BRLF1 locus, overlapping with BRLF1 but in inverse orientation. We have characterized the 5' end of the BRRF1 mRNA and the promoter, PNa, at which BRRF1 pre-mRNA is initiated. We show that although a single BRRF1 mRNA species is induced by 12-O-tetradecanoylphorbol 13-acetate/sodium butyrate in several EBV-infected B cell lines, in Akata cells treated with anti-IgG two BRRF1 mRNAs can be detected. Transcription initiated at the BRRF1 promoter was activated by EB1 but not by R, and EB1-binding sites which contribute to the EB1-activated transcription have been mapped to between positions -469 and +1. A 34 kDa protein could be translated from the BRRF1 mRNA both in vitro and in vivo, and was found predominantly in the nucleus of HeLa cells transfected with a BRRF1 expression vector. Thus there are three promoters in the region of the EBV chromatin containing the BZLF1/BRLF1 genes, two of which, PZ and PNa, potentially share regulatory elements.

This article has been cited by other articles:

				S. Lee, H.-J. Cho, J.-J. Park, Y.-S. Kim, S. Hwang, R. Sun, and M. J. Song The ORF49 Protein of Murine Gammaherpesvirus 68 Cooperates with RTA in Regulating Virus Replication J. Virol., September 15, 2007; 81(18): 9870 - 9877. [Abstract] [Full Text] [PDF]

				C. M. Gonzalez, E. L. Wong, B. S. Bowser, G. K. Hong, S. Kenney, and B. Damania Identification and Characterization of the Orf49 Protein of Kaposi's Sarcoma-Associated Herpesvirus J. Virol., March 15, 2006; 80(6): 3062 - 3070. [Abstract] [Full Text] [PDF]

				G. K. Hong, H.-J. Delecluse, H. Gruffat, T. E. Morrison, W.-H. Feng, A. Sergeant, and S. C. Kenney The BRRF1 Early Gene of Epstein-Barr Virus Encodes a Transcription Factor That Enhances Induction of Lytic Infection by BRLF1 J. Virol., May 15, 2004; 78(10): 4983 - 4992. [Abstract] [Full Text] [PDF]

				H. Gruffat, J. Batisse, D. Pich, B. Neuhierl, E. Manet, W. Hammerschmidt, and A. Sergeant Epstein-Barr Virus mRNA Export Factor EB2 Is Essential for Production of Infectious Virus J. Virol., August 28, 2002; 76(19): 9635 - 9644. [Abstract] [Full Text] [PDF]