HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Donofrio, G. Articles by van Santen, V. L. Search for Related Content PubMed PubMed Citation Articles by Donofrio, G. Articles by van Santen, V. L. Agricola Articles by Donofrio, G. Articles by van Santen, V. L.

Establishment of a cell line persistently infected with bovine herpesvirus-4 by use of a recombinant virus

Istituto di Malattie Infettive Veterinarie, Facoltà di Medicina Veterinaria, Università degli Studi di Parma, 43100 Parma, Italy¹
Department of Pathobiology, 264 Greene Hall, College of Veterinary Medicine, Auburn University, Auburn, AL 36849-5519, USA²

Author for correspondence: Vicky van Santen. Fax +1 334 844 2652. e-mail vvsanten{at}mail.auburn.edu

Bovine herpesvirus-4 (BHV-4), a gammaherpesvirus lacking a clear disease association, productively infects multiple cell lines of various species and causes cell death. A human rhabdomyosarcoma cell line, RD-4, infected with BHV-4 produced low levels of early and late viral RNAs and infectious virus, but exhibited no cytopathic effect. Using a recombinant BHV-4 containing a neomycin-resistance gene, we established RD-4-derived cell lines persistently infected with BHV-4. The viral genome in these cells was predominantly circular. Because of drug selection, every cell contained a viral genome. In addition, all cells stained with a BHV-4-specific antiserum. Therefore, these cell lines are not carrier cultures. These cells produced infectious virus at all passages tested. Even though cells were selected and maintained at a concentration of geneticin at least 2·5 times that necessary to kill uninfected RD-4 cells, selected cells contained only approximately one viral genome per diploid host cell genome. Persistently infected cells grew more slowly than uninfected cells, even in the absence of drug. The slower growth of these cells suggests that any growth advantage conferred by multiple copies of the neomycin-gene-carrying viral genome might be offset by the detrimental effects of viral gene expression. This situation contrasts with other gammaherpesviruses, which are able to growth-transform cells.

This article has been cited by other articles:

				G. Donofrio, S. Herath, C. Sartori, S. Cavirani, C. F. Flammini, and I. M. Sheldon Bovine herpesvirus 4 is tropic for bovine endometrial cells and modulates endocrine function Reproduction, July 1, 2007; 134(1): 183 - 197. [Abstract] [Full Text] [PDF]

				G. Donofrio, S. Cavirani, A. Vanderplasschen, L. Gillet, and C. F. Flammini Recombinant Bovine Herpesvirus 4 (BoHV-4) Expressing Glycoprotein D of BoHV-1 Is Immunogenic and Elicits Serum-Neutralizing Antibodies against BoHV-1 in a Rabbit Model Clin. Vaccine Immunol., November 1, 2006; 13(11): 1246 - 1254. [Abstract] [Full Text] [PDF]

				L. Gillet, B. Dewals, F. Farnir, L. de Leval, and A. Vanderplasschen Bovine Herpesvirus 4 Induces Apoptosis of Human Carcinoma Cell Lines In vitro and In vivo Cancer Res., October 15, 2005; 65(20): 9463 - 9472. [Abstract] [Full Text] [PDF]

				G. Donofrio, S. Cavirani, V. van Santen, and C. F. Flammini Potential Secondary Pathogenic Role for Bovine Herpesvirus 4 J. Clin. Microbiol., July 1, 2005; 43(7): 3421 - 3426. [Abstract] [Full Text] [PDF]

				G. Donofrio, F. L. Heppner, M. Polymenidou, C. Musahl, and A. Aguzzi Paracrine Inhibition of Prion Propagation by Anti-PrP Single-Chain Fv Miniantibodies J. Virol., July 1, 2005; 79(13): 8330 - 8338. [Abstract] [Full Text] [PDF]

				L. Gillet, F. Minner, B. Detry, F. Farnir, L. Willems, M. Lambot, E. Thiry, P.-P. Pastoret, F. Schynts, and A. Vanderplasschen Investigation of the Susceptibility of Human Cell Lines to Bovine Herpesvirus 4 Infection: Demonstration that Human Cells Can Support a Nonpermissive Persistent Infection Which Protects Them against Tumor Necrosis Factor Alpha-Induced Apoptosis J. Virol., March 1, 2004; 78(5): 2336 - 2347. [Abstract] [Full Text] [PDF]

				G. Donofrio and V. L. van Santen A bovine macrophage cell line supports bovine herpesvirus-4 persistent infection J. Gen. Virol., May 1, 2001; 82(5): 1181 - 1185. [Abstract] [Full Text]

				G. Donofrio, C. F. Flammini, F. Scatozza, and S. Cavirani Detection of Bovine Herpesvirus 4 (BoHV-4) DNA in the Cell Fraction of Milk of Dairy Cattle with History of BoHV-4 Infection J. Clin. Microbiol., December 1, 2000; 38(12): 4668 - 4671. [Abstract] [Full Text]