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Journal of General Virology (2000), 81, 1857-1864.
© 2000 Society for General Microbiology

Plant

Potato leafroll virus protein P1 contains a serine proteinase domain

X. Li1, M. D. Ryan1 and J. W. Lamb1

Centre for Biomolecular Sciences, University of St Andrews, North Haugh, St Andrews KY16 9ST, UK1

Author for correspondence: Martin Ryan. Fax +44 1334 462595. e-mail martin.ryan{at}st-and.ac.uk

The multi-domain potato leafroll virus replicase protein P1 was expressed in insect cells from the polyhedrin promoter of Autographa californica nucleopolyhedrovirus. Using antisera raised against P1, it was shown that P1 was cleaved near the VPg in insect cells in a manner similar to that in plant cells, to produce a ~27 kDa C-terminal fragment. Furthermore, it was shown that the proposed serine proteinase-like domain within P1 is responsible for this processing and that this can occur in a trans (intermolecular) reaction. Four conserved residues within the serine proteinase domain that are essential for catalysis have been identified, consistent with the proposal that this domain comprises a serine proteinase.




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