HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Westra, D. F. Articles by Welling-Wester, S. Search for Related Content PubMed PubMed Citation Articles by Westra, D. F. Articles by Welling-Wester, S. Agricola Articles by Westra, D. F. Articles by Welling-Wester, S.

Natural infection with herpes simplex virus type 1 (HSV-1) induces humoral and T cell responses to the HSV-1 glycoprotein H:L complex

Department of Medical Microbiology¹ and Department of Clinical Immunology⁴, University of Groningen, Hanzeplein 1, 9713 GZ Groningen, The Netherlands
Rotterdam Eye Hospital² and Institute of Virology³, Erasmus University Rotterdam, Rotterdam, The Netherlands

Author for correspondence: Sytske Welling-Wester. Fax +31 503633528. e-mail S.Welling-Wester{at}med.rug.nl

The glycoproteins of herpes simplex virus type 1 (HSV-1) are important targets for the immune system in the control of HSV-1 infections. The humoral and T cell responses to the glycoprotein (g)H_t(His):gL complex of HSV-1 were studied in seven HSV-1-seropositive and three HSV-1-seronegative healthy adults. In addition, responses to HSV-1 gD_t were determined. As antigens, purified soluble recombinant forms of the gH_t(His):gL complex produced by insect cells and of gD_t produced by yeast cells were used. In contrast to seronegative donors, sera of all seropositive donors contained gH_t(His): gL-specific IgG. Using peripheral blood (PB) T cells, gH_t(His):gL-specific proliferative T cell responses were detected in all seropositive donors. Culture supernatants of PB T cells stimulated with recombinant gH_t(His):gL contained high levels of interferon- and no detectable interleukin-4, indicating their Th1 phenotype. These results show that naturally acquired HSV-1 infection induces gH:gL-specific humoral and T cell responses.

This article has been cited by other articles:

				L. H. Herbst, S. Lemaire, A. R. Ene, D. J. Heslin, L. M. Ehrhart, D. A. Bagley, P. A. Klein, and J. Lenz Use of Baculovirus-Expressed Glycoprotein H in an Enzyme-Linked Immunosorbent Assay Developed To Assess Exposure to Chelonid Fibropapillomatosis-Associated Herpesvirus and Its Relationship to the Prevalence of Fibropapillomatosis in Sea Turtles Clin. Vaccine Immunol., May 1, 2008; 15(5): 843 - 851. [Abstract] [Full Text] [PDF]

				D. M. Koelle and L. Corey Recent Progress in Herpes Simplex Virus Immunobiology and Vaccine Research Clin. Microbiol. Rev., January 1, 2003; 16(1): 96 - 113. [Abstract] [Full Text] [PDF]

				D. M. Koelle, M. Schomogyi, C. McClurkan, S. N. Reymond, and H. B. Chen CD4 T-Cell Responses to Herpes Simplex Virus Type 2 Major Capsid Protein VP5: Comparison with Responses to Tegument and Envelope Glycoproteins J. Virol., December 1, 2000; 74(23): 11422 - 11425. [Abstract] [Full Text]