HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Chang, J. Articles by Ganem, D. Search for Related Content PubMed PubMed Citation Articles by Chang, J. Articles by Ganem, D. Agricola Articles by Chang, J. Articles by Ganem, D.

On the control of late gene expression in Kaposi’s sarcoma-associated herpesvirus (human herpesvirus-8)

Howard Hughes Medical Institute and Departments of Microbiology and Medicine, University of California, San Francisco, CA 94143-0414, USA¹

Author for correspondence: Don Ganem. Fax +1 415 476 0939. e-mail ganem{at}socrates.ucsf.edu

Herpesvirus late genes require viral DNA replication for maximal expression. Although late gene expression appears to require DNA replication in cis in alphaherpesviruses, studies in Epstein–Barr virus (EBV) suggest that this cis-requirement might not pertain to the gammaherpesviruses. Based on these findings, a system was created to investigate the elements required for the regulation of Kaposi’s sarcoma-associated herpesvirus (KSHV; human herpesvirus-8) late gene expression. The transcript of a classic late gene encoding the viral assembly protein was characterized and reporter genes driven by the assembly protein promoter region were constructed. Unlike the EBV case, expression of a reporter gene under the control of the assembly protein promoter did not display authentic regulation when removed from the context of the viral genome. Although reporter expression rose in cells displaying lytic replication, this expression was not diminished by specific inhibitors of viral DNA synthesis. Minimal core promoters were similarly unable to reproduce late gene regulation. These results suggest that proper KSHV late gene expression is likely to be dependent upon virus lytic replication in cis and indicate that the regulation of KSHV late genes more closely resembles that observed in herpes simplex virus than that described for EBV.

This article has been cited by other articles:

				E. Wong, T.-T. Wu, N. Reyes, H. Deng, and R. Sun Murine Gammaherpesvirus 68 Open Reading Frame 24 Is Required for Late Gene Expression after DNA Replication J. Virol., June 15, 2007; 81(12): 6761 - 6764. [Abstract] [Full Text] [PDF]

				J. Ziegelbauer, A. Grundhoff, and D. Ganem Exploring the DNA Binding Interactions of the Kaposi's Sarcoma-Associated Herpesvirus Lytic Switch Protein by Selective Amplification of Bound Sequences In Vitro J. Virol., March 15, 2006; 80(6): 2958 - 2967. [Abstract] [Full Text] [PDF]

				J. Bechtel, A. Grundhoff, and D. Ganem RNAs in the Virion of Kaposi's Sarcoma-Associated Herpesvirus J. Virol., August 15, 2005; 79(16): 10138 - 10146. [Abstract] [Full Text] [PDF]

				B. Damania, J. H. Jeong, B. S. Bowser, S. M. DeWire, M. R. Staudt, and D. P. Dittmer Comparison of the Rta/Orf50 Transactivator Proteins of Gamma-2-Herpesviruses J. Virol., May 15, 2004; 78(10): 5491 - 5499. [Abstract] [Full Text] [PDF]

				S. Tang, K. Yamanegi, and Z.-M. Zheng Requirement of a 12-Base-Pair TATT-Containing Sequence and Viral Lytic DNA Replication in Activation of the Kaposi's Sarcoma-Associated Herpesvirus K8.1 Late Promoter J. Virol., March 1, 2004; 78(5): 2609 - 2614. [Abstract] [Full Text] [PDF]

				C. M. O'Connor, B. Damania, and D. H. Kedes De Novo Infection with Rhesus Monkey Rhadinovirus Leads to the Accumulation of Multiple Intranuclear Capsid Species during Lytic Replication but Favors the Release of Genome-Containing Virions J. Virol., December 15, 2003; 77(24): 13439 - 13447. [Abstract] [Full Text] [PDF]

				S. Tang and Z.-M. Zheng Kaposi's Sarcoma-associated Herpesvirus K8 Exon 3 Contains Three 5'-Splice Sites and Harbors a K8.1 Transcription Start Site J. Biol. Chem., April 19, 2002; 277(17): 14547 - 14556. [Abstract] [Full Text] [PDF]

				K. Nealon, W. W. Newcomb, T. R. Pray, C. S. Craik, J. C. Brown, and D. H. Kedes Lytic Replication of Kaposi's Sarcoma-Associated Herpesvirus Results in the Formation of Multiple Capsid Species: Isolation and Molecular Characterization of A, B, and C Capsids from a Gammaherpesvirus J. Virol., March 15, 2001; 75(6): 2866 - 2878. [Abstract] [Full Text]