HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Zhang, Y.-J. Articles by Gao, S.-J. Search for Related Content PubMed PubMed Citation Articles by Zhang, Y.-J. Articles by Gao, S.-J. Agricola Articles by Zhang, Y.-J. Articles by Gao, S.-J.

Hot-spot variations of Kaposi’s sarcoma-associated herpesvirus latent nuclear antigen and application in genotyping by PCR–RFLP

Departments of Pediatrics and Microbiology, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229-3900, USA¹
National Cancer Institute, Bethesda, Maryland, USA²

Author for correspondence: Shou-Jiang Gao. Fax +1 210 567 6305. e-mail gaos{at}uthscsa.edu

Kaposi’s sarcoma-associated herpesvirus (KSHV, human herpesvirus-8) is aetiologically associated with Kaposi’s sarcoma and several other lymphoproliferative disorders. The latent nuclear antigen (LNA) encoded by KSHV ORF73 has important functions in virus latent infection and shows molecular polymorphism. Sequence variations were identified in the internal repeat domain (IRD) of ORF73. DNA sequencing of ORF73 from one KSHV-infected cell line, PK-1, revealed that there were 558 bp (30·2%) deletions and 66 (3·6%) point mutations located mainly in repeat region 2, the glutamine-rich region of ORF73 IRD, compared with ORF73 of BC-1 KSHV. Similar sequence variations of ORF73 were also identified in two other isolates. None of the sequence variations caused any translational frame-shift in these four KSHV isolates examined, suggesting that LNA has a conservative function in virus latent infection. The frequent sequence variations in repeat region 2 of ORF73 IRD were also identified by PCR–RFLP genotyping in 26 KSHV isolates, suggesting that this region is a ‘hot-spot’ for genetic variations. Each Kaposi’s sarcoma lesion sample contained one virus genotype with a unique RFLP pattern, indicating that in vivo KSHV infection was established with single predominate genotypes, which was further supported by the presence of invariable genotypes in multifocal lesions from individual KS patients. Four KSHV subtypes were classified based on the RFLP patterns that represent the patterns of DNA sequence variations in the ORF73 IRD. PCR–RFLP genotyping is capable of identifying LNA genetic variations and differentiating individual KSHV isolates, and thus may be useful for KSHV molecular epidemiology studies.

This article has been cited by other articles:

				J. Hart, M. Ackermann, G. Jayawardane, G. Russell, D. M. Haig, H. Reid, and J. P. Stewart Complete sequence and analysis of the ovine herpesvirus 2 genome J. Gen. Virol., January 1, 2007; 88(1): 28 - 39. [Abstract] [Full Text] [PDF]

				N. S. Taus, D. R. Herndon, D. L. Traul, J. P. Stewart, M. Ackermann, H. Li, D. P. Knowles, G. S. Lewis, and K. A. Brayton Comparison of ovine herpesvirus 2 genomes isolated from domestic sheep (Ovis aries) and a clinically affected cow (Bos bovis) J. Gen. Virol., January 1, 2007; 88(1): 40 - 45. [Abstract] [Full Text] [PDF]

				J. L. Taylor, H. N. Bennett, B. A. Snyder, P. S. Moore, and Y. Chang Transcriptional Analysis of Latent and Inducible Kaposi's Sarcoma-Associated Herpesvirus Transcripts in the K4 to K7 Region J. Virol., December 15, 2005; 79(24): 15099 - 15106. [Abstract] [Full Text] [PDF]

				F.-C. Ye, F.-C. Zhou, S. M. Yoo, J.-P. Xie, P. J. Browning, and S.-J. Gao Disruption of Kaposi's Sarcoma-Associated Herpesvirus Latent Nuclear Antigen Leads to Abortive Episome Persistence J. Virol., October 15, 2004; 78(20): 11121 - 11129. [Abstract] [Full Text] [PDF]

				L. A. Dourmishev, A. L. Dourmishev, D. Palmeri, R. A. Schwartz, and D. M. Lukac Molecular Genetics of Kaposi's Sarcoma-Associated Herpesvirus (Human Herpesvirus 8) Epidemiology and Pathogenesis Microbiol. Mol. Biol. Rev., June 1, 2003; 67(2): 175 - 212. [Abstract] [Full Text] [PDF]

				L. J. Coulter and H. W. Reid Isolation and expression of three open reading frames from ovine herpesvirus-2 J. Gen. Virol., March 1, 2002; 83(3): 533 - 543. [Abstract] [Full Text] [PDF]

				T. Piolot, M. Tramier, M. Coppey, J.-C. Nicolas, and V. Marechal Close but Distinct Regions of Human Herpesvirus 8 Latency-Associated Nuclear Antigen 1 Are Responsible for Nuclear Targeting and Binding to Human Mitotic Chromosomes J. Virol., April 15, 2001; 75(8): 3948 - 3959. [Abstract] [Full Text]