| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Animal: RNA Viruses |
Gladstone Institute of Virology and Immunology, PO Box 419100, San Francisco, CA 94141-9100, USA1
Department of Medicine, School of Medicine, University of California San Francisco, San Francisco, CA 94141-9100, USA2
Author for correspondence: Mark Goldsmith (at Gladstone Institute of Virology and Immunology). Fax +1 415 695 1364. e-mail mgoldsmith{at}gladstone.ucsf.edu
Human infection by Marburg (MBG) or Ebola (EBO) virus is associated with fatal haemorrhagic fevers. While these filoviruses may both incite disease as a result of explosive virus replication, we hypothesized that expression of individual viral gene products, such as the envelope glycoprotein (GP), may directly alter target cells and contribute to pathogenesis. We found that expression of EBO GP in 293T cells caused significant levels of cellular detachment in the absence of cell death or virus replication. This detachment was induced most potently by membrane-bound EBO GP, rather than the shed glycoprotein products (sGP or GP1), and was largely attributable to a domain within the extracellular region of GP2. Furthermore, detachment was blocked by the Ser/Thr kinase inhibitor 2-aminopurine, suggesting the importance of a phosphorylation-dependent signalling cascade in inducing detachment. Since MBG GP did not induce similar cellular detachment, MBG and EBO GP interact with target cells by distinct processes to elicit cellular dysregulation.
This article has been cited by other articles:
|
|
 |
|
  St. P. Reid, C. Valmas, O. Martinez, F. M. Sanchez, and C. F. Basler Ebola Virus VP24 Proteins Inhibit the Interaction of NPI-1 Subfamily Karyopherin {alpha} Proteins with Activated STAT1 J. Virol., December 15, 2007; 81(24): 13469 - 13477. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. A. Zampieri, J.-F. Fortin, G. P. Nolan, and G. J. Nabel The ERK Mitogen-Activated Protein Kinase Pathway Contributes to Ebola Virus Glycoprotein-Induced Cytotoxicity J. Virol., February 1, 2007; 81(3): 1230 - 1240. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
St. P. Reid, L. W. Leung, A. L. Hartman, O. Martinez, M. L. Shaw, C. Carbonnelle, V. E. Volchkov, S. T. Nichol, and C. F. Basler Ebola Virus VP24 Binds Karyopherin {alpha}1 and Blocks STAT1 Nuclear Accumulation. J. Virol., June 1, 2006; 80(11): 5156 - 5167. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Alazard-Dany, V. Volchkova, O. Reynard, C. Carbonnelle, O. Dolnik, M. Ottmann, A. Khromykh, and V. E. Volchkov Ebola virus glycoprotein GP is not cytotoxic when expressed constitutively at a moderate level. J. Gen. Virol., May 1, 2006; 87(Pt 5): 1247 - 1257. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Bar, A. Takada, Y. Kawaoka, and M. Alizon Detection of Cell-Cell Fusion Mediated by Ebola Virus Glycoproteins J. Virol., March 15, 2006; 80(6): 2815 - 2822. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. M. Wahl-Jensen, T. A. Afanasieva, J. Seebach, U. Stroher, H. Feldmann, and H.-J. Schnittler Effects of Ebola Virus Glycoproteins on Endothelial Cell Activation and Barrier Function J. Virol., August 15, 2005; 79(16): 10442 - 10450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Wahl-Jensen, S. K. Kurz, P. R. Hazelton, H.-J. Schnittler, U. Stroher, D. R. Burton, and H. Feldmann Role of Ebola Virus Secreted Glycoproteins and Virus-Like Particles in Activation of Human Macrophages J. Virol., February 15, 2005; 79(4): 2413 - 2419. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. J. Sullivan, M. Peterson, Z.-y. Yang, W.-p. Kong, H. Duckers, E. Nabel, and G. J. Nabel Ebola Virus Glycoprotein Toxicity Is Mediated by a Dynamin-Dependent Protein-Trafficking Pathway J. Virol., January 1, 2005; 79(1): 547 - 553. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Watanabe, T. Watanabe, T. Noda, A. Takada, H. Feldmann, L. D. Jasenosky, and Y. Kawaoka Production of Novel Ebola Virus-Like Particles from cDNAs: an Alternative to Ebola Virus Generation by Reverse Genetics J. Virol., January 15, 2004; 78(2): 999 - 1005. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Simmons, R. J. Wool-Lewis, F. Baribaud, R. C. Netter, and P. Bates Ebola Virus Glycoproteins Induce Global Surface Protein Down-Modulation and Loss of Cell Adherence J. Virol., March 1, 2002; 76(5): 2518 - 2528. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Feldmann, V. E. Volchkov, V. A. Volchkova, U. Stroher, and H.-D. Klenk Biosynthesis and role of filoviral glycoproteins J. Gen. Virol., December 1, 2001; 82(12): 2839 - 2848. [Full Text] [PDF]
|
|
|
|
|
|
U. Stroher, E. West, H. Bugany, H.-D. Klenk, H.-J. Schnittler, and H. Feldmann Infection and Activation of Monocytes by Marburg and Ebola Viruses J. Virol., November 15, 2001; 75(22): 11025 - 11033. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ito, S. Watanabe, A. Takada, and Y. Kawaoka Ebola Virus Glycoprotein: Proteolytic Processing, Acylation, Cell Tropism, and Detection of Neutralizing Antibodies J. Virol., February 1, 2001; 75(3): 1576 - 1580. [Abstract] [Full Text]
|
|
|
|
 |
|
 V. E. Volchkov, V. A. Volchkova, E. Mühlberger, L. V. Kolesnikova, M. Weik, O. Dolnik, and H.-D. Klenk Recovery of Infectious Ebola Virus from Complementary DNA: RNA Editing of the GP Gene and Viral Cytotoxicity Science, March 9, 2001; 291(5510): 1965 - 1969. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
