HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ishikawa, M. Articles by Ahlquist, P. Search for Related Content PubMed PubMed Citation Articles by Ishikawa, M. Articles by Ahlquist, P. Agricola Articles by Ishikawa, M. Articles by Ahlquist, P.

The 3a cell-to-cell movement gene is dispensable for cell-to-cell transmission of brome mosaic virus RNA replicons in yeast but retained over 10⁴⁵-fold amplification

Institute for Molecular Virology¹ and Howard Hughes Medical Institute², University of Wisconsin–Madison, Madison, Wisconsin 53706, USA

Author for correspondence: Masayuki Ishikawa. Present address: Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan. Fax +81 11 706 4932. e-mail ishikawa{at}abs.agr.ac.jp

In yeast expressing the RNA replication proteins encoded by brome mosaic virus (BMV), B3URA3, a BMV RNA3 derivative that harbours the 3a cell-to-cell movement protein gene and the yeast uracil biosynthesis gene URA3, was replicated and maintained in 85–95% of progeny at each cell division. Transmission of the B3URA3 RNA replicon from mother to daughter yeast did not require the 3a gene. Nevertheless, even after passaging for 165 cycles of RNA replication and yeast cell division, each of 40 independent Ura⁺ colonies tested retained B3URA3 RNAs whose electrophoretic mobilities and accumulation levels were indistinguishable from those of the original B3URA3. These and other results suggest that unselected genes in many positive-strand RNA virus replicons can be stably retained if the presence of the gene does not confer a selective disadvantage in RNA replication.

This article has been cited by other articles:

				C. J. Beckham, H. R. Light, T. Amar Nissan, P. Ahlquist, R. Parker, and A. Noueiry Interactions between Brome Mosaic Virus RNAs and Cytoplasmic Processing Bodies J. Virol., September 15, 2007; 81(18): 9759 - 9768. [Abstract] [Full Text] [PDF]

				H. Garcia-Ruiz and P. Ahlquist Inducible Yeast System for Viral RNA Recombination Reveals Requirement for an RNA Replication Signal on Both Parental RNAs. J. Virol., September 1, 2006; 80(17): 8316 - 8328. [Abstract] [Full Text] [PDF]