| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Animal: RNA Viruses |
Laboratoire d’Epidémiologie Moléculaire des Entérovirus, Institut Pasteur, 25 rue du Dr Roux, 75724 Paris Cedex 15, France1
Author for correspondence: Valérie Caro. Fax +33 1 45 68 87 80. e-mail vcaro{at}pasteur.fr
To explore further the phylogenetic relationships between human enteroviruses and to develop new diagnostic approaches, we designed a pair of generic primers in order to study a 1452 bp genomic fragment (relative to the poliovirus Mahoney genome), including the 3' end of the VP1-coding region, the 2A- and 2B-coding regions, and the 5' moiety of the 2C-coding region. Fifty-nine of the 64 prototype strains and 45 field isolates of various origins, involving 21 serotypes and 6 strains untypeable by standard immunological techniques, were successfully amplified with these primers. By determining the nucleotide sequence of the genomic fragment encoding the C-terminal third of the VP1 capsid protein we developed a molecular typing method based on RT–PCR and sequencing. If field isolate sequences were compared to human enterovirus VP1 sequences available in databases, nucleotide identity score was, in each case, highest with the homotypic prototype (74.8 to 89.4%). Phylogenetic trees were generated from alignments of partial VP1 sequences with several phylogeny algorithms. In all cases, the new classification of enteroviruses into five identified species was confirmed and strains of the same serotype were always monophyletic. Analysis of the results confirmed that the 3' third of the VP1-coding sequence contains serotype-specific information and can be used as the basis of an effective and rapid molecular typing method. Furthermore, the amplification of such a long genomic fragment, including non-structural regions, is straightforward and could be used to investigate genome variability and to identify recombination breakpoints or specific attributes of pathogenicity.
This article has been cited by other articles:
|
|
 |
|
  B. A. Brown, K. Maher, M. R. Flemister, P. Naraghi-Arani, M. Uddin, M. S. Oberste, and M. A. Pallansch Resolving ambiguities in genetic typing of human enterovirus species C clinical isolates and identification of enterovirus 96, 99 and 102 J. Gen. Virol., July 1, 2009; 90(7): 1713 - 1723. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. C. M. Leitch, J. Bendig, M. Cabrerizo, J. Cardosa, T. Hyypia, O. E. Ivanova, A. Kelly, A. C. M. Kroes, A. Lukashev, A. MacAdam, et al. Transmission Networks and Population Turnover of Echovirus 30 J. Virol., March 1, 2009; 83(5): 2109 - 2118. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Blomqvist, A. Paananen, C. Savolainen-Kopra, T. Hovi, and M. Roivainen Eight Years of Experience with Molecular Identification of Human Enteroviruses J. Clin. Microbiol., July 1, 2008; 46(7): 2410 - 2413. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Mirand, C. Henquell, C. Archimbaud, M. Chambon, F. Charbonne, H. Peigue-Lafeuille, and J.-L. Bailly Prospective Identification of Enteroviruses Involved in Meningitis in 2006 through Direct Genotyping in Cerebrospinal Fluid J. Clin. Microbiol., January 1, 2008; 46(1): 87 - 96. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Dedepsidis, Z. Kyriakopoulou, V. Pliaka, C. Kottaridi, E. Bolanaki, S. Levidiotou-Stefanou, D. Komiotis, and P. Markoulatos Retrospective Characterization of a Vaccine-Derived Poliovirus Type 1 Isolate from Sewage in Greece Appl. Envir. Microbiol., November 1, 2007; 73(21): 6697 - 6704. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Nasri, L. Bouslama, S. Omar, H. Saoudin, T. Bourlet, M. Aouni, B. Pozzetto, and S. Pillet Typing of Human Enterovirus by Partial Sequencing of VP2 J. Clin. Microbiol., August 1, 2007; 45(8): 2370 - 2379. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Simmonds Recombination and Selection in the Evolution of Picornaviruses and Other Mammalian Positive-Stranded RNA Viruses J. Virol., November 15, 2006; 80(22): 11124 - 11140. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. N. Zhao, D. S. Perlin, S. Park, R. J. Jiang, L. Chen, Y. Chen, R. Gardiner, and Q. W. Jiang FDJS03 Isolates Causing an Outbreak of Aseptic Meningitis in China That Evolved from a Distinct Echovirus 30 Lineage Imported from Countries of the Commonwealth of Independent States J. Clin. Microbiol., November 1, 2006; 44(11): 4142 - 4148. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. A. Nix, M. S. Oberste, and M. A. Pallansch Sensitive, Seminested PCR Amplification of VP1 Sequences for Direct Identification of All Enterovirus Serotypes from Original Clinical Specimens. J. Clin. Microbiol., August 1, 2006; 44(8): 2698 - 2704. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Richter, D. Koptides, C. Tryfonos, and C. Christodoulou Molecular typing of enteroviruses associated with viral meningitis in Cyprus, 2000-2002. J. Med. Microbiol., August 1, 2006; 55(Pt 8): 1035 - 1041. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Kyriakopoulou, C. Kottaridi, E. Dedepsidis, E. Bolanaki, S. Levidiotou-Stefanou, and P. Markoulatos Molecular Characterization of Wild-Type Polioviruses Isolated in Greece during the 1996 Outbreak in Albania. J. Clin. Microbiol., March 1, 2006; 44(3): 1150 - 1152. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Arita, S.-L. Zhu, H. Yoshida, T. Yoneyama, T. Miyamura, and H. Shimizu A Sabin 3-Derived Poliovirus Recombinant Contained a Sequence Homologous with Indigenous Human Enterovirus Species C in the Viral Polymerase Coding Region J. Virol., October 15, 2005; 79(20): 12650 - 12657. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Korimbocus, N. Scaramozzino, B. Lacroix, J. M. Crance, D. Garin, and G. Vernet DNA Probe Array for the Simultaneous Identification of Herpesviruses, Enteroviruses, and Flaviviruses J. Clin. Microbiol., August 1, 2005; 43(8): 3779 - 3787. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Rakoto-Andrianarivelo, D. Rousset, R. Razafindratsimandresy, S. Chevaliez, S. Guillot, J. Balanant, and F. Delpeyroux High Frequency of Human Enterovirus Species C Circulation in Madagascar J. Clin. Microbiol., January 1, 2005; 43(1): 242 - 249. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Heim From poliovirus surveillance to enterovirus surveillance: a complete picture? J. Med. Microbiol., January 1, 2005; 54(1): 1 - 2. [Full Text] [PDF]
|
|
|
|
|
|
O Bahri, D Rezig, B B. Nejma-Oueslati, A B. Yahia, J B. Sassi, N Hogga, A Sadraoui, and H Triki Enteroviruses in Tunisia: virological surveillance over 12 years (1992-2003) J. Med. Microbiol., January 1, 2005; 54(1): 63 - 69. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Oberste, S. M. Michele, K. Maher, D. Schnurr, D. Cisterna, N. Junttila, M. Uddin, J.-J. Chomel, C.-S. Lau, W. Ridha, et al. Molecular identification and characterization of two proposed new enterovirus serotypes, EV74 and EV75 J. Gen. Virol., November 1, 2004; 85(11): 3205 - 3212. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Thoelen, E. Moes, P. Lemey, S. Mostmans, E. Wollants, A. M. Lindberg, A.-M. Vandamme, and M. Van Ranst Analysis of the Serotype and Genotype Correlation of VP1 and the 5' Noncoding Region in an Epidemiological Survey of the Human Enterovirus B Species J. Clin. Microbiol., March 1, 2004; 42(3): 963 - 971. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Costa-Mattioli, A. D. Napoli, V. Ferre, S. Billaudel, R. Perez-Bercoff, and J. Cristina Genetic variability of hepatitis A virus J. Gen. Virol., December 1, 2003; 84(12): 3191 - 3201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. N. Lukashev, V. A. Lashkevich, O. E. Ivanova, G. A. Koroleva, A. E. Hinkkanen, and J. Ilonen Recombination in Circulating Enteroviruses J. Virol., October 1, 2003; 77(19): 10423 - 10431. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Brown, M. S. Oberste, K. Maher, and M. A. Pallansch Complete Genomic Sequencing Shows that Polioviruses and Members of Human Enterovirus Species C Are Closely Related in the Noncapsid Coding Region J. Virol., August 15, 2003; 77(16): 8973 - 8984. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Norder, L. Bjerregaard, L. Magnius, B. Lina, M. Aymard, and J.-J. Chomel Sequencing of 'untypable' enteroviruses reveals two new types, EV-77 and EV-78, within human enterovirus type B and substitutions in the BC loop of the VP1 protein for known types J. Gen. Virol., April 1, 2003; 84(4): 827 - 836. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Blomqvist, A.-L. Bruu, M. Stenvik, and T. Hovi Characterization of a recombinant type 3/type 2 poliovirus isolated from a healthy vaccinee and containing a chimeric capsid protein VP1 J. Gen. Virol., March 1, 2003; 84(3): 573 - 580. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-R. Wang, H.-P. Tsai, S.-W. Huang, P.-H. Kuo, D. Kiang, and C.-C. Liu Laboratory Diagnosis and Genetic Analysis of an Echovirus 30-Associated Outbreak of Aseptic Meningitis in Taiwan in 2001 J. Clin. Microbiol., December 1, 2002; 40(12): 4439 - 4444. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Manzara, M. Muscillo, G. La Rosa, C. Marianelli, P. Cattani, and G. Fadda Molecular Identification and Typing of Enteroviruses Isolated from Clinical Specimens J. Clin. Microbiol., December 1, 2002; 40(12): 4554 - 4560. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Oprisan, M. Combiescu, S. Guillot, V. Caro, A. Combiescu, F. Delpeyroux, and R. Crainic Natural genetic recombination between co-circulating heterotypic enteroviruses J. Gen. Virol., September 1, 2002; 83(9): 2193 - 2200. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Yin, A.-K. Berg, T. Tuvemo, and G. Frisk Enterovirus RNA Is Found in Peripheral Blood Mononuclear Cells in a Majority of Type 1 Diabetic Children at Onset Diabetes, June 1, 2002; 51(6): 1964 - 1971. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Palacios, I. Casas, D. Cisterna, G. Trallero, A. Tenorio, and C. Freire Molecular Epidemiology of Echovirus 30: Temporal Circulation and Prevalence of Single Lineages J. Virol., April 16, 2002; 76(10): 4940 - 4949. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. S. Oberste, K. Maher, and M. A. Pallansch Molecular Phylogeny and Proposed Classification of the Simian Picornaviruses J. Virol., February 1, 2002; 76(3): 1244 - 1251. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. A. Bendig, P. S. O'Brien, and P. Muir Serotype-Specific Detection of Coxsackievirus A16 in Clinical Specimens by Reverse Transcription-Nested PCR J. Clin. Microbiol., October 1, 2001; 39(10): 3690 - 3692. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
