HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Song, B. H. Articles by Lee, C. H. Search for Related Content PubMed PubMed Citation Articles by Song, B. H. Articles by Lee, C. H. Agricola Articles by Song, B. H. Articles by Lee, C. H.

Human cytomegalovirus binding to heparan sulfate proteoglycans on the cell surface and/or entry stimulates the expression of human leukocyte antigen class I

Division of Life Sciences, College of Natural Sciences¹ and Research Institute for Genetic Engineering², Chungbuk National University, Cheongju, Chungbuk 361-763, South Korea

Author for correspondence: Chan Hee Lee at Division of Life Sciences, College of Natural Sciences. Fax +82 43 273 2451. e-mail chlee{at}cbucc.chungbuk.ac.kr

Human cytomegalovirus (HCMV) is known to down-regulate the expression of human leukocyte antigen (HLA) class I, the process of which involves a subset of virus genes. Infection of human foreskin fibroblast (HFF) cells with UV-inactivated HCMV (UV-HCMV), however, resulted in an increase in HLA class I presentation on the cell surface in the absence of HCMV gene expression. Heparin, which inhibits the interaction of virus particles with cell surface heparan sulfate proteoglycans (HSPGs), blocked the effect of UV-HCMV on HLA class I expression. Pretreatment of cells with heparinase I decreased in a dose-dependent manner the effect of UV-HCMV on HLA class I expression enhancement. Sodium chlorate, which is known to inhibit the sulfation of HSPGs, gave a similar result. Pretreatment of UV-HCMV with trypsin or monoclonal antibody reactive with the envelope glycoprotein gB reduced the increase in HLA class I expression on the HFF cell surface by UV-HCMV. RT–PCR analysis demonstrated that the increase in HLA class I presentation on the HFF cell surface was due to an increase in HLA class I transcription. Thus, binding of HCMV particles to cell surface HSPGs appears to be required for the stimulation of HLA class I expression. It is also possible that virus entry, in addition to binding to HSPGs, may be involved in the stimulation of HLA class I expression, since the UV-HCMV entered the cells and all treatments to block virus binding to HSPGs would necessarily prevent virus entry.

This article has been cited by other articles:

				H. F. Vischer, J. C. M. Granneman, and J. Bogerd Identification of Follicle-Stimulating Hormone-Selective {beta}-Strands in the N-Terminal Hormone-Binding Exodomain of Human Gonadotropin Receptors Mol. Endocrinol., August 1, 2006; 20(8): 1880 - 1893. [Abstract] [Full Text] [PDF]

				H. Jenssen, P. Hamill, and R. E. W. Hancock Peptide Antimicrobial Agents Clin. Microbiol. Rev., July 1, 2006; 19(3): 491 - 511. [Abstract] [Full Text] [PDF]

				K. S. Jones, C. Petrow-Sadowski, D. C. Bertolette, Y. Huang, and F. W. Ruscetti Heparan Sulfate Proteoglycans Mediate Attachment and Entry of Human T-Cell Leukemia Virus Type 1 Virions into CD4+ T Cells J. Virol., October 15, 2005; 79(20): 12692 - 12702. [Abstract] [Full Text] [PDF]

				G. Pollara, M. Jones, M. E. Handley, M. Rajpopat, A. Kwan, R. S. Coffin, G. Foster, B. Chain, and D. R. Katz Herpes Simplex Virus Type-1-Induced Activation of Myeloid Dendritic Cells: The Roles of Virus Cell Interaction and Paracrine Type I IFN Secretion J. Immunol., September 15, 2004; 173(6): 4108 - 4119. [Abstract] [Full Text] [PDF]