| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Animal: DNA Viruses |
Laboratory of Viral Oncology, Division of Immunology, Allergy and Infectious Diseases (DIAID)1, and Division of General Dermatology, Department of Dermatology2, University of Vienna Medical School, Währinger Gürtel 18–20, A-1090 Vienna, Austria
Center for Ultrastructure Research and Ludwig Boltzmann Institute for Molecular Nanotechnology, University of Agricultural Sciences, Gregor Mendel Strasse 33, A-1180 Vienna, Austria3
Author for correspondence: Reinhard Kirnbauer. Fax +43 1 403 1900. e-mail reinhard.kirnbauer{at}akh-wien.ac.at
Neutralization capsid epitopes are important determinants for antibody-mediated immune protection against papillomavirus (PV) infection and induced disease. Chimeric L1 major capsid proteins of the human PV type 16 (HPV-16) and the bovine PV type 1 (BPV-1) with a foreign peptide incorporated into several capsid surface loops self-assembled into pentamers or virus-like particles (VLP). Binding patterns of neutralizing monoclonal antibodies (MAb) and immunization of mice confirmed (i) that regions around aa 282–286 and 351–355 contribute to neutralization epitopes and identified the latter region as an immunodominant site and (ii) that placing a foreign peptide in the context of an assembled structure markedly enhanced its immunogenicity. Pentamers disassembled from wild-type HPV-16 and BPV-1 VLPs displayed some of the neutralization epitopes that were detected on fully assembled VLPs, but were deficient for binding a subset of neutralizing MAb that inhibit cell attachment.
This article has been cited by other articles:
|
|
 |
|
  C. Schellenbacher, R. Roden, and R. Kirnbauer Chimeric L1-L2 Virus-Like Particles as Potential Broad-Spectrum Human Papillomavirus Vaccines J. Virol., October 1, 2009; 83(19): 10085 - 10095. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Handisurya, R. Gambhira, C. Schellenbacher, S. Shafti-Keramat, O. Forslund, M. Favre, and R. Kirnbauer Serological relationship between cutaneous human papillomavirus types 5, 8 and 92 J. Gen. Virol., January 1, 2009; 90(1): 136 - 143. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Bishop, J. Dasgupta, M. Klein, R. L. Garcea, N. D. Christensen, R. Zhao, and X. S. Chen Crystal Structures of Four Types of Human Papillomavirus L1 Capsid Proteins: UNDERSTANDING THE SPECIFICITY OF NEUTRALIZING MONOCLONAL ANTIBODIES J. Biol. Chem., October 26, 2007; 282(43): 31803 - 31811. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Ryding, L. Dahlberg, M. Wallen-Ohman, and J. Dillner Deletion of a major neutralizing epitope of human papillomavirus type 16 virus-like particles J. Gen. Virol., March 1, 2007; 88(3): 792 - 802. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Zamora, A. Handisurya, S. Shafti-Keramat, D. Borchelt, G. Rudow, K. Conant, C. Cox, J. C. Troncoso, and R. Kirnbauer Papillomavirus-Like Particles Are an Effective Platform for Amyloid-beta Immunization in Rabbits and Transgenic Mice J. Immunol., August 15, 2006; 177(4): 2662 - 2670. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-N. Billaud, D. Peterson, M. Barr, A. Chen, M. Sallberg, F. Garduno, P. Goldstein, W. McDowell, J. Hughes, J. Jones, et al. Combinatorial Approach to Hepadnavirus-Like Particle Vaccine Design J. Virol., November 1, 2005; 79(21): 13656 - 13666. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Shafti-Keramat, A. Handisurya, E. Kriehuber, G. Meneguzzi, K. Slupetzky, and R. Kirnbauer Different Heparan Sulfate Proteoglycans Serve as Cellular Receptors for Human Papillomaviruses J. Virol., December 15, 2003; 77(24): 13125 - 13135. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Varsani, A.-L. Williamson, D. de Villiers, I. Becker, N. D. Christensen, and E. P. Rybicki Chimeric Human Papillomavirus Type 16 (HPV-16) L1 Particles Presenting the Common Neutralizing Epitope for the L2 Minor Capsid Protein of HPV-6 and HPV-16 J. Virol., August 1, 2003; 77(15): 8386 - 8393. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-M. Wang, J. C. Cook, J. C. Lee, K. U. Jansen, N. D. Christensen, S. W. Ludmerer, and W. L. McClements Human papillomavirus type 6 virus-like particles present overlapping yet distinct conformational epitopes J. Gen. Virol., June 1, 2003; 84(6): 1493 - 1497. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
