HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Touzé, A. Articles by Coursaget, P. Search for Related Content PubMed PubMed Citation Articles by Touzé, A. Articles by Coursaget, P. Agricola Articles by Touzé, A. Articles by Coursaget, P.

Gene transfer using human polyomavirus BK virus-like particles expressed in insect cells

Laboratoire de Virologie Moléculaire, EMI-U 00-10 Protéases et Vectorisation, IFR Transposons et Virus, Faculté des Sciences Pharmaceutiques ‘Philippe Maupas’, 31 avenue Monge, 37200 Tours, France¹
Laboratoires de Biologie Cellulaire et Virologie, EA 2639, Analyse Structurale des Antigènes, IFR Transposons et Virus, Faculté de Médecine, 2 bis Boulevard Tonnellé, 37032 Tours cedex, France²

Author for correspondence: Pierre Coursaget. Fax +33 2 47 36 71 88. e-mail coursaget{at}univ-tours.fr

The major structural protein (VP1) of the BK polyomavirus (BKV) was expressed in the recombinant baculovirus expression system. Recombinant BKV VP1 was shown to self-assemble into virus-like particles (VLPs) with a diameter of 45–50 nm. As for other polyomaviruses, BKV VP1 has the capacity to bind to exogenous DNA. Furthermore, the potential of BKV VP1 VLPs was investigated for gene transfer into COS-7 cells using three methods for the formation of pseudo-virions: disassembly/reassembly, osmotic shock and direct interaction between VLPs and reporter plasmid DNA. The latter method was shown to be the most efficient when using linearized plasmid. Gene transfer efficiency with BKV pseudo-virions was of the same order as that observed with human papillomavirus type 16 L1 protein VLPs. In addition, it is demonstrated that cellular entry of BKV pseudo-virions is dependent on cell surface sialic acid.

This article has been cited by other articles:

				H. Tsukamoto, M.-a. Kawano, T. Inoue, T. Enomoto, R.-u Takahashi, N. Yokoyama, N. Yamamoto, T. Imai, K. Kataoka, Y. Yamaguchi, et al. Evidence that SV40 VP1-DNA interactions contribute to the assembly of 40-nm spherical viral particles. Genes Cells, November 1, 2007; 12(11): 1267 - 1279. [Abstract] [Full Text] [PDF]

				N. Yokoyama, M.-a. Kawano, H. Tsukamoto, T. Enomoto, T. Inoue, R.-u Takahashi, A. Nakanishi, T. Imai, T. Wada, and H. Handa Mutational Analysis of the Carboxyl-terminal Region of the SV40 Major Capsid Protein VP1 J. Biochem., February 1, 2007; 141(2): 279 - 286. [Abstract] [Full Text] [PDF]

				L. Bousarghin, A. Touze, A.-L. Combita-Rojas, and P. Coursaget Positively charged sequences of human papillomavirus type 16 capsid proteins are sufficient to mediate gene transfer into target cells via the heparan sulfate receptor J. Gen. Virol., January 1, 2003; 84(1): 157 - 164. [Abstract] [Full Text] [PDF]

				A.-L. Combita, A. Touze, L. Bousarghin, N. D. Christensen, and P. Coursaget Identification of Two Cross-Neutralizing Linear Epitopes within the L1 Major Capsid Protein of Human Papillomaviruses J. Virol., June 5, 2002; 76(13): 6480 - 6486. [Abstract] [Full Text] [PDF]