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Animal: DNA Viruses |
Department of Animal Health and Well-being, Faculty of Veterinary Medicine of Bari, S.p. per Casamassima km 3, 70010 Valenzano, Bari, Italy1
Department of Pathology and Infectious Diseases, University of Messina, Messina, Italy2
James Baker Institute, Cornell University, Ithaca, NY, USA3
Author for correspondence: Canio Buonavoglia. Fax +39 080 4679843. e-mail c.buonavoglia{at}veterinaria.uniba.it
Two isolates of canine parvovirus (CPV) were obtained from dogs affected with severe haemorrhagic diarrhoea. Type 2b antigenic specificity was predicted by both antigenic analysis with monoclonal antibodies and PCR characterization with type-specific primers. Nevertheless, sequence analysis of the capsid protein-encoding gene revealed two amino acid changes. One of the changes affected position 426 (Asp to Glu), in a major antigenic site of the viral capsid, determining the replacement of a residue unique to CPV type 2b. The failure of established typing methods to distinguish this antigenic variant was overcome by the development of an RFLP assay.
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