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Insect |
United States Department of Agriculture, Agricultural Research Service, Center for Medical, Agricultural, and Veterinary Entomology, 1600/1700 SW 23rd Drive, Gainesville, FL 32604, USA
USDA/ARS Plum Island Animal Disease Center, Plum Island, New York, USA2
Sequencing Core Facility of the ICBR, University of Florida, Gainesville, Florida, USA3
Author for correspondence: Bettina Moser. Fax +1 352 374 5922. e-mail bmoser{at}gainesville.usda.ufl.edu
We present evidence that a newly discovered mosquito virus from Culex nigripalpus is an unusual member of the family Baculoviridae. Development of this virus was restricted to nuclei of midgut epithelial cells in the gastric caeca and posterior stomach. The globular occlusion bodies were not enveloped, measured around 400 nm in diameter, occurred exclusively in nuclei of infected cells and typically contained four, sometimes up to eight, virions. The developmental sequence involved two virion phenotypes: an occluded form (ODV) that initiated infection in the midgut epithelial cells, and a budded form that spread the infection in the midgut. Each ODV contained one rod-shaped enveloped nucleocapsid (40x200 nm). The double-stranded DNA genome was approximately 105–110 kbp with an estimated GC content of 52%. We have sequenced approximately one-third of the genome and detected 96 putative ORFs of 50 amino acids or more including several genes considered to be unique to baculoviruses. Phylogenetic analysis of the amino acid sequences of DNApol and p74 placed this virus in a separate clade from the genera Nucleopolyhedrovirus and Granulovirus. We provisionally assign this virus in the genus Nucleopolyhedrovirus, henceforth abbreviated as CuniNPV (for Culex nigripalpus nucleopolyhedrovirus), and suggest that, awaiting additional data to clarify its taxonomic status, it may be a member of a new genus within the family Baculoviridae.
This article has been cited by other articles:
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 S. C. Braunagel, W. K. Russell, G. Rosas-Acosta, D. H. Russell, and M. D. Summers Determination of the protein composition of the occlusion-derived virus of Autographa californica nucleopolyhedrovirus PNAS, August 19, 2003; 100(17): 9797 - 9802. [Abstract] [Full Text] [PDF]
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C.-H. Cheng, S.-M. Liu, T.-Y. Chow, Y.-Y. Hsiao, D.-P. Wang, J.-J. Huang, and H.-H. Chen Analysis of the Complete Genome Sequence of the Hz-1 Virus Suggests that It Is Related to Members of the Baculoviridae J. Virol., August 12, 2002; 76(18): 9024 - 9034. [Abstract] [Full Text] [PDF]
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V. S. Mikhailov and G. F. Rohrmann Baculovirus Replication Factor LEF-1 Is a DNA Primase J. Virol., March 1, 2002; 76(5): 2287 - 2297. [Abstract] [Full Text] [PDF]
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C. L. Afonso, E. R. Tulman, Z. Lu, C. A. Balinsky, B. A. Moser, J. J. Becnel, D. L. Rock, and G. F. Kutish Genome Sequence of a Baculovirus Pathogenic for Culex nigripalpus J. Virol., November 15, 2001; 75(22): 11157 - 11165. [Abstract] [Full Text] [PDF]
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 J. M. Slack, E. M. Dougherty, and S. D. Lawrence A study of the Autographa californica multiple nucleopolyhedrovirus ODV envelope protein p74 using a GFP tag J. Gen. Virol., September 1, 2001; 82(9): 2279 - 2287. [Abstract] [Full Text] [PDF]
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J. J. Becnel, S. E. White, B. A. Moser, T. Fukuda, M. J. Rotstein, A. H. Undeen, and A. Cockburn Epizootiology and transmission of a newly discovered baculovirus from the mosquitoes Culex nigripalpus and C. quinquefasciatus J. Gen. Virol., February 1, 2001; 82(2): 275 - 282. [Abstract] [Full Text]
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