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Divergent replication kinetics of two phenotypically different parvoviruses of rats

Section of Comparative Medicine, Yale University School of Medicine, PO Box 208016, CT 06520-8016, New Haven, USA¹

Author for correspondence: Lisa Ball-Goodrich. Fax +1 203 785 7499. e-mail lisa.ball-goodrich{at}yale.edu

Rat virus (RV) is an important infectious agent of laboratory rats because of its high prevalence and capacity to disrupt research. Additionally, RV infection serves as a model for characterizing virus–host interactions during acute, persistent and prenatal infection. Our research has examined the pathogenesis of two RV strains, RV-UMass and RV-Y. RV-UMass is more pathogenic, causes a higher level of persistent infection and transmits to the foetus after oronasal inoculation of the pregnant dam. To determine in vitro distinctions between the strains that may account for these differences and to provide a benchmark for characterizing virus replication in vivo, synchronized in vitro replication of both RV strains was defined and compared. The results demonstrated that RV replication has replicative intermediates, virus transcripts and proteins similar to those reported for the prototype parvovirus, minute virus of mice. However, the replicative cycle of RV-UMass was 12 h compared with 24 h for RV-Y, and RV-UMass and RV-Y differed in kinetics of virus DNA replication, transcription and protein accumulation. Additionally, in situ analysis correlated well with kinetics data as determined by Southern and Northern blot analysis. Sequence comparisons between the strains also determined coding differences that may contribute to phenotypic differences.

This article has been cited by other articles:

				L. J. Ball-Goodrich, F. X. Paturzo, E. A. Johnson, K. Steger, and R. O. Jacoby Immune Responses to the Major Capsid Protein during Parvovirus Infection of Rats J. Virol., August 28, 2002; 76(19): 10044 - 10049. [Abstract] [Full Text] [PDF]