HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Frischmuth, T. Articles by Kocher, C. Search for Related Content PubMed PubMed Citation Articles by Frischmuth, T. Articles by Kocher, C. Agricola Articles by Frischmuth, T. Articles by Kocher, C.

The size of encapsidated single-stranded DNA determines the multiplicity of African cassava mosaic virus particles

Biologisches Institut, Abteilung für Molekularbiologie und Virologie der Pflanzen, Universität Stuttgart, Pfaffenwaldring 57, 70550 Stuttgart, Germany¹

Author for correspondence: Thomas Frischmuth. Fax +49 711 6855096. e-mail thomas.frischmuth{at}po.uni-stuttgart.de

Transgenic Nicotiana benthamiana plants harbouring a defective interfering (DI) DNA of African cassava mosaic virus (ACMV) and control plants were inoculated with ACMV. Virus particles were purified from infected plants, separated in sucrose gradients and fractions were analysed by Southern blotting. Transgenic plant-derived virus particles taken from the top fractions of sucrose gradients contained DI DNA, middle fractions contained a mixture of genomic and DI DNA and bottom fractions contained a mixture of multimeric, genomic and DI DNA. Virus particles from selected top, middle and bottom fractions were analysed by electron microscopy. In fractions containing only DI DNA, isometric particles of 18–20 nm were detected. In fractions containing DI DNA as well as genomic size DNA, isometric and geminate particles were found. Fractions containing multimeric size DNA were found to comprise particles consisting of three subunits adjacent to geminate particles. From these data, it is concluded that the size of encapsidated DNA determines the multiplicity of ACMV particles.

This article has been cited by other articles:

				P. Caciagli, V. Medina Piles, D. Marian, M. Vecchiati, V. Masenga, G. Mason, T. Falcioni, and E. Noris Virion Stability Is Important for the Circulative Transmission of Tomato Yellow Leaf Curl Sardinia Virus by Bemisia tabaci, but Virion Access to Salivary Glands Does Not Guarantee Transmissibility J. Virol., June 1, 2009; 83(11): 5784 - 5795. [Abstract] [Full Text] [PDF]

				S. Hehnle, C. Wege, and H. Jeske Interaction of DNA with the Movement Proteins of Geminiviruses Revisited J. Virol., July 15, 2004; 78(14): 7698 - 7706. [Abstract] [Full Text] [PDF]

				B. Bottcher, S. Unseld, H. Ceulemans, R. B. Russell, and H. Jeske Geminate Structures of African Cassava Mosaic Virus J. Virol., July 1, 2004; 78(13): 6758 - 6765. [Abstract] [Full Text] [PDF]