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Virology Department, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK1
Graduate School of Biotechnology, Korea University, Seoul 136-701, Republic of Korea2
Author for correspondence: Peter Palukaitis. Fax +44 1382 562426. e-mail ppaluk{at}scri.sari.ac.uk
Tobacco plants transgenic for RNA 1 of Cucumber mosaic virus and inoculated with transcript of RNAs 2 and 3 regenerated viral RNA 1 from the transgenic mRNA, and the plants became systemically infected by the reconstituted virus. cDNA fragments corresponding to the 3' non-coding region (NCR) of viral RNA 1 were amplified, cloned and sequenced. In some clones the termini of the 3' NCR corresponded to those of viral RNAs 2 or 3. This suggested that in some cases RNA 1 may have been regenerated during replication by a template switching mechanism between the inoculated transcript RNAs and the mRNA. However, encapsidated, recombinant RNA 1 with the 3' NCR ends originating from RNAs 2 or 3 also was found in virus samples that had been passaged exclusively through non-transgenic plants. Thus, these chimeras occur naturally due to recombination between wild-type viral RNAs, and they are found encapsidated in low, but detectable amounts.
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