HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Canto, T. Articles by Palukaitis, P. Search for Related Content PubMed PubMed Citation Articles by Canto, T. Articles by Palukaitis, P. Agricola Articles by Canto, T. Articles by Palukaitis, P.

A subpopulation of RNA 1 of Cucumber mosaic virus contains 3' termini originating from RNAs 2 or 3

Virology Department, Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, UK¹
Graduate School of Biotechnology, Korea University, Seoul 136-701, Republic of Korea²

Author for correspondence: Peter Palukaitis. Fax +44 1382 562426. e-mail ppaluk{at}scri.sari.ac.uk

Tobacco plants transgenic for RNA 1 of Cucumber mosaic virus and inoculated with transcript of RNAs 2 and 3 regenerated viral RNA 1 from the transgenic mRNA, and the plants became systemically infected by the reconstituted virus. cDNA fragments corresponding to the 3' non-coding region (NCR) of viral RNA 1 were amplified, cloned and sequenced. In some clones the termini of the 3' NCR corresponded to those of viral RNAs 2 or 3. This suggested that in some cases RNA 1 may have been regenerated during replication by a template switching mechanism between the inoculated transcript RNAs and the mRNA. However, encapsidated, recombinant RNA 1 with the 3' NCR ends originating from RNAs 2 or 3 also was found in virus samples that had been passaged exclusively through non-transgenic plants. Thus, these chimeras occur naturally due to recombination between wild-type viral RNAs, and they are found encapsidated in low, but detectable amounts.

This article has been cited by other articles:

				B.-J. Shi, R. H. Symons, and P. Palukaitis The cucumovirus 2b gene drives selection of inter-viral recombinants affecting the crossover site, the acceptor RNA and the rate of selection Nucleic Acids Res., March 27, 2008; 36(4): 1057 - 1071. [Abstract] [Full Text] [PDF]

				R. Wierzchoslawski and J. J. Bujarski Efficient In Vitro System of Homologous Recombination in Brome Mosaic Bromovirus. J. Virol., June 1, 2006; 80(12): 6182 - 6187. [Abstract] [Full Text] [PDF]

				S. K. Choi, P. Palukaitis, B. E. Min, M. Y. Lee, J. K. Choi, and K. H. Ryu Cucumber mosaic virus 2a polymerase and 3a movement proteins independently affect both virus movement and the timing of symptom development in zucchini squash J. Gen. Virol., April 1, 2005; 86(4): 1213 - 1222. [Abstract] [Full Text] [PDF]

				H.-X. Lin, L. Rubio, A. B. Smythe, and B. W. Falk Molecular Population Genetics of Cucumber Mosaic Virus in California: Evidence for Founder Effects and Reassortment J. Virol., June 15, 2004; 78(12): 6666 - 6675. [Abstract] [Full Text] [PDF]

				D. van Meerten, H. Groeneveld, D. M. J. Miller, G. B. Marechal, N. V. Tsareva, R. C. L. Olsthoorn, M. de la Pena, and J. van Duin In vivo generation of hybrids between different species of RNA phages J. Gen. Virol., May 1, 2002; 83(5): 1223 - 1235. [Abstract] [Full Text] [PDF]

				Y.-K. Chen, R. Goldbach, and M. Prins Inter- and Intramolecular Recombinations in the Cucumber Mosaic Virus Genome Related to Adaptation to Alstroemeria J. Virol., March 19, 2002; 76(8): 4119 - 4124. [Abstract] [Full Text] [PDF]